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Transcript

An Easy to draw microscope

Instructions for using a microscope.

Notebook Template

Only write down the highlighted labels.

Arm

Stage Clip

Coarse Knob

Fine Knob

Stage

Objective Lens

Eyepiece

This is the part of the microscope that you look through. It contains 10x magnification.

Only pick the microscope up by this handle. Use your other hand underneath the microscope for more support.

Use these clips to hold your slide in place and keep it from moving around.

This is also known as the course knob. It is what moves the stage up and down. You adjust this knob before the small one.

Also known as the fine focus knob, this does not move the stage, but it will change the focus by a small amount. This should only be used once the specimen is almost in focus and just needs a little boost.

The stage is where you will place your slide. It can be moved up or down with the coarse knob in order to bring your specimen into focus.

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Basic Instructions for Using the Light Microscope Prepare the Workspace: Ensure you have a clean, well-lit, and stable surface for setting up the microscope. Keep the area organized and free from clutter. Turn On the Light Source: If your microscope has an adjustable light source, switch it on. Adjust the brightness to an optimal level for viewing the specimen. Place the Slide: Place the prepared slide on the stage of the microscope. Secure it in place using the mechanical stage clips or slide holder. Use the Lowest Objective Lens: Start with the lowest magnification objective lens (usually 4x or 10x) by rotating the nosepiece to position it over the slide. Adjust the Focus: Look through the eyepiece and slowly turn the coarse focus knob to bring the specimen into a rough focus. Then, use the fine focus knob for precise focusing. Continue adjusting until the image is clear. Change to Higher Magnification: If needed, switch to higher magnification lenses (40x or 100x) by rotating the nosepiece. Be cautious with higher magnifications as they have a smaller depth of field and require more precise adjustments. Center the Specimen: Adjust the mechanical stage controls to move the slide and center the area of interest within the field of view.

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These lenses come in three different magnifications. 4x, 10x, and 40x. It can be rotated to switch between objective lenses. They should "click" into place.

Objective Lenses (Magnification)

  • Start with the lowest magnification.
  • Rotate the lenses to switch between them. They should "click" into place.
  • If your image gets "lost" at a higher magnification, lower the magnifcation to find your image, and then try icreasing it again.

In order to view a specimen under the microscope you will need a slide and coverslip.

Preparing a specimen.

  • Place your specimen on a glass slide.
  • Cover it with a coverslip.
  • Be careful, but if anything breaks, make sure to let your instructor know immediately.

slide

coverslip