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Microscope

Lauren Cruz

Created on November 28, 2023

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Eyepiece

An Easy to draw microscope

Instructions for using a microscope.

Arm

Notebook Template

Objective Lens

Stage

Stage Clip

Coarse Knob

Fine Knob

Visit noun project for more microscope images.

thenounproject.com

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Basic Instructions for Using the Light Microscope Prepare the Workspace: Ensure you have a clean, well-lit, and stable surface for setting up the microscope. Keep the area organized and free from clutter. Turn On the Light Source: If your microscope has an adjustable light source, switch it on. Adjust the brightness to an optimal level for viewing the specimen. Place the Slide: Place the prepared slide on the stage of the microscope. Secure it in place using the mechanical stage clips or slide holder. Use the Lowest Objective Lens: Start with the lowest magnification objective lens (usually 4x or 10x) by rotating the nosepiece to position it over the slide. Adjust the Focus: Look through the eyepiece and slowly turn the coarse focus knob to bring the specimen into a rough focus. Then, use the fine focus knob for precise focusing. Continue adjusting until the image is clear. Change to Higher Magnification: If needed, switch to higher magnification lenses (40x or 100x) by rotating the nosepiece. Be cautious with higher magnifications as they have a smaller depth of field and require more precise adjustments. Center the Specimen: Adjust the mechanical stage controls to move the slide and center the area of interest within the field of view.

Back

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Objective Lenses (Magnification)

These lenses come in three different magnifications. 4x, 10x, and 40x. It can be rotated to switch between objective lenses. They should "click" into place.

  • Start with the lowest magnification.
  • Rotate the lenses to switch between them. They should "click" into place.
  • If your image gets "lost" at a higher magnification, lower the magnifcation to find your image, and then try icreasing it again.
Preparing a specimen.

In order to view a specimen under the microscope you will need a slide and coverslip.

coverslip

  • Place your specimen on a glass slide.
  • Cover it with a coverslip.
  • Be careful, but if anything breaks, make sure to let your instructor know immediately.

slide