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FBXW7

Kellie Krohn

Created on May 4, 2023

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Protein Project: FBXW7

Advanced Biochemistry Dr. Fishovitz Kellie Krohn

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Why should we study cancer?

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Around 2,000,000 Americans are diagnosed with cancer each year. Around 600,000 Americans will pass away due to cancer in just one year.

Research

Studying proteins may increase our understanding on what mechanisms drive cancer

F-Box W7 Protein

FBXW7 function

belonging to the Skp1-Cdc53/Cullin-F-box-protein complex (SCF/β-TrCP)- F-Box proteins- regulate proteins involved in DNA replication, transcription, cell differentiation, and cell death, via ubiquitination-mediated degradation of target proteins (Kahn, 2021).

FBXW7 role in humans

often inactivated or mutated in humans- correlates with:- cancer progression - therapy resistance - poor prognosis Indicated as a potential target for treatment for many cancers

Research Techniques:

Cellular Assays

Proliferation Assay- How a molecule may affect a cells proliferation, growth or viability
Wound Scratch Assay- How a molecule affects migration of the cells
Chemotaxis Assay- Whether cells migrate towards a particular agent

Chemotaxis Assay

Proliferation assay

Scratch Wound Assay

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Endometrial Cancer Cell Lines

Future Directions with studying FBXW7

Additional cell lines: HEC-1-B, HEC-1-A, KLE, SKOV3 shRNA knockouts, western blots

Current Research on FBXW7

FBXW7 role in:- Notch Signaling Pathway and Hepatic Fibrosis - Colorectal Cancer

The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China

Eureka!

Thank you!!

Refrences

Cuevas, I. C.; Sahoo, S. S.; Kumar, A.; Zhang, H.; Westcott, J.; Aguilar, M.; Cortez, J. D.; Sullivan, S. A.; Xing, C.; Hayes, D. N.; Brekken, R. A.; Bae-Jump, V. L.; Castrillon, D. H. Fbxw7 Is a Driver of Uterine Carcinosarcoma by Promoting Epithelial-Mesenchymal Transition. Proceedings of the National Academy of Sciences 2019, 116 (51), 25880-25890. https://doi.org/10.1073/pnas.1911310116.Liu, Y., Chen, H., Bao, H., Zhang, J., Wu, R., & Zhu, L. (2023). Comprehensive characterization of FBXW7 mutational and clinicopathological profiles in human colorectal cancers. Frontiers in oncology, 13, 1154432. https://doi.org/10.3389/fonc.2023.1154432Sun, Y., He, L., Guo, P., Li, F., Wang, B., Zhang, Y., An, K., & Peng, M. (2023). F-box and WD repeat domain containing 7 inhibits the activation of hepatic stellate cells by degrading delta-like ligand 1 to block Notch signaling pathway. Open medicine (Warsaw, Poland), 18(1), 20230634. https://doi.org/10.1515/med-2023-0634

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Comparison

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Timeline

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