K.O/K.I
Delitto Perfetto
Big Picture
K.I Counter-selection
Part 2: Knock-in
K.O Selection
Part 1: Knockout
vertical STAIRs INFOGRAPHIC
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Did you know that besides taking advantage of our resources, you can add any visual elements you want to complement the information? Try it from the Image or Resources menus!
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Do you need more reasons to create dynamic content? Well: 90% of the information we assimilate comes to us through sight, and moreover, we retain 42% more information when the content is moving.
Not just any old sm
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Multimedia content is essential in a presentation to leave everyone speechless. Moreover, this way you will synthesize the content and keep your audience entertained.
Write a great headline
If you want to provide additional information or develop the content in more detail, you can do so through your oral presentation. We recommend that you practice your voice and rehearse: the best improvisation is always the most prepared!
Write a great headline
Here you can put a highlighted title, something that captivates your audience.
Did you know that in addition to taking advantage of our resources, you can add any visual elements you want to complement the information? Try it from the Image or Resources menus! Pose a dramatic question; it is the essential ingredient to keep the audience's attention. It is usually posed subtly at the beginning of the story to intrigue the audience and is resolved at the end.
Write a great headline
With the tool, creating infographics is faster and easier than making a brownie in the microwave. You can refer to the graphs available in the Resources section.
Write a great headline
Do you need more reasons to create dynamic content? Well: 90% of the information we assimilate comes to us through sight, and we retain 42% more information when the content is moving.
How a K.O Works
The goal is to repace a WT gene/gene of interest with a selectable marker (SM). This SM is on a plasmid and will have primers and homology arms (H.As) added to it to create the K.O PCR SM fragment. During homologous directed recombination (HDR), the K.O occurs!
The Knock-in process
Now that the SM is in the endogenous location of the WT gene and we've confirmed the K.O, we can K.I! The same process will occur. A plasmid with the gene/mutant of interest(G/MOI) undergoes PCR with primers and H.As. This amplicon is then added in during transformation. Goal: GOI/MOI knocks into place!
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K.O/K.I
Janhavi Joshi
Created on April 19, 2026
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Transcript
K.O/K.I
Delitto Perfetto
Big Picture
K.I Counter-selection
Part 2: Knock-in
K.O Selection
Part 1: Knockout
vertical STAIRs INFOGRAPHIC
Write a great headline
Write a great headline
Write a great headline
Write a great headline
Write a great headline
Write a great headline
Write a great headline
Write a great headline
Write a great headline
Did you know that besides taking advantage of our resources, you can add any visual elements you want to complement the information? Try it from the Image or Resources menus!
Write a great headline
Do you need more reasons to create dynamic content? Well: 90% of the information we assimilate comes to us through sight, and moreover, we retain 42% more information when the content is moving.
Not just any old sm
Write a great headline
Multimedia content is essential in a presentation to leave everyone speechless. Moreover, this way you will synthesize the content and keep your audience entertained.
Write a great headline
If you want to provide additional information or develop the content in more detail, you can do so through your oral presentation. We recommend that you practice your voice and rehearse: the best improvisation is always the most prepared!
Write a great headline
Here you can put a highlighted title, something that captivates your audience.
Did you know that in addition to taking advantage of our resources, you can add any visual elements you want to complement the information? Try it from the Image or Resources menus! Pose a dramatic question; it is the essential ingredient to keep the audience's attention. It is usually posed subtly at the beginning of the story to intrigue the audience and is resolved at the end.
Write a great headline
With the tool, creating infographics is faster and easier than making a brownie in the microwave. You can refer to the graphs available in the Resources section.
Write a great headline
Do you need more reasons to create dynamic content? Well: 90% of the information we assimilate comes to us through sight, and we retain 42% more information when the content is moving.
How a K.O Works
The goal is to repace a WT gene/gene of interest with a selectable marker (SM). This SM is on a plasmid and will have primers and homology arms (H.As) added to it to create the K.O PCR SM fragment. During homologous directed recombination (HDR), the K.O occurs!
The Knock-in process
Now that the SM is in the endogenous location of the WT gene and we've confirmed the K.O, we can K.I! The same process will occur. A plasmid with the gene/mutant of interest(G/MOI) undergoes PCR with primers and H.As. This amplicon is then added in during transformation. Goal: GOI/MOI knocks into place!
Write a great headline