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Central dogma of molecular biology

Yessika Andrea Orea Yañez

Created on October 29, 2025

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Transcript

attendance

FUNDAMENTALS OF LIFE

reminders

Tomorrow 31 oct: 1. Strategic activity 7 2. Quiz 4 Monday 3 november: 1. Prelab 3 Tuesday 4 november: 1. Lab practice (bring labcoat, dresscode, evidence sheet, MATERIALS: 2 BANANAS AND 6 STRAWBERRIES FOR ALL THE GROUP)

Module 8. How can mutations in genes influence the function of a living organism?

Nucleic acids recap

Which where the nucleic acids?

Monomer

Build-a-Nucleotide Challenge!

1. You will be given a card. 2. Move around the classrom as the music plays. 3. When the music stops get together with you classmates to form a nucleotide (sugar, phosphate, nitogenous base)

Build-a-Nucleotide Challenge!

1. Move around the classrom as the music plays. 2. When the music stops get together with you classmates to form a nucleotide (sugar, phosphate, nitogenous base) 3. If the teacher says "CHAIN" you need to form nucleotides and join with the nucleotides of the corresponding nucleic acid.

In DNA to form the double helix... Bases pair with each other:

C G T A

"se jetea"

Moving on

lets remember some concepts

Compact organization of genes."Packages" of genetic material.

CHROMOSOME

It is the unit of genetic information (where the "instructions" for making an organism are).

DNA

GENE

It is a segment of DNA that encodes a specific protein/function.

Moving on

lets remember some concepts

Compact organization of genes."Packages" of genetic material.

CHROMOSOME

It is the unit of genetic information (where the "instructions" for making an organism are).

DNA

GENE

It is a segment of DNA that encodes a specific protein/function.

How do we go from a DNA gene to a protein?

Central Dogma of Molecular Biology

REPLICATION

To replicate or duplicate genetic material means to make a copy of the genetic material so that there are two identical copies.

remember the S phase?

DNA molecule

The double helix unwinds. An enzyme called DNA helicase decompresses the DNA so that the two strands are separated.

Each original strand serves as a template for the formation of a new strand. DNA polymerase fills the strands with new DNA. One strand is synthesized continuously, and the other is synthesized discontinuously.

DNA ligase joins the fragments of the discontinuous strand. The two daughter DNA molecules will be identical to the parent molecule.

Replication is "SEMICONSERVATIVE" because each copy contains one old strand and one new strand.

EXERCISE

ATG CCG TTA GCA TTA GCG TAC GAC GGA CTT AAG

TRANSCRIPTION

We go from DNA (deoxyribonucleic acid) to RNA (ribonucleic acid)

ARN TYPES

MESSENGER RNA

RNA carries the genetic information from DNA out of the nucleus and begins to produce proteins. Depending on the type of cell, the proteins produced and the quantity vary.

Takes place in the nucleus: 1. A segment of DNA serves as a template for the production of a molecule of mRNA. 2. The DNA helix opens up so that complementary base pairing can occur between the DNA and the mRNA. 3. RNA polymerase (an enzyme) joins new RNA nucleotides in a sequence complementary to that of the DNA.

EXERCISE

Main chain

ATG CCG TTA GCA TTA GCG TAC GAC GGA CTT AAG

Daughter chain

TAC GGC AAC CGT AAC CGC ATG CTC CCT GAA TTC

TRANSLATION

We go from RNA (ribonucleic acid) to proteins

Ribosomes decode mRNA by reading its nucleotides in groups of three, called codons. During TRANSLATION, the tRNA has anticodons whose bases pair with a codon in the mRNA. The codons correspond to a specific amino acid. Three codons signal the end of a protein. A "start" codon, AUG, marks the beginning of a protein.

AUG GUU AUU CUU UAA Met Val Ile Leu Stop

EXERCISE

Main chain

ATG CCG TTA GCA TTA GCG TAC GAC GGA CTT AAG

Daughter chain

TAC GGC AAC CGT AAC CGC ATG CTC CCT GAA TTC

AUG CCG UUA GCA UUA GCG UAC GAC GGA CUU AAG

EXERCISE

AUG CCG UUA GCA UUA GCG UAC GAC GGA CUU AAG