safe storage of samples in liquid nitrogen

Safe storage of samples in liquid nitrogen

Main hazards

• Explosion of vials due to liquid nitrogen leakage.
• Injuries, biological exposure, extreme cold, asphyxiation.

📌 Avoid storing in liquid phase unless necessary.

Protective clothing and equipment

• Long-sleeved lab coat.
• Cryogenic gloves.
• Full face shield.
• Long trousers and closed shoes.

Equipment and accessories

• Dewar certified: check for damages.
• Vials: only suitable for cryogenic use. Preferably store in the vapour phase.
• Sealing: resistance up to -196 °C.

Best practices

• Fill vials to a maximum of 85% .
• Disinfect the outside before inserting them.
• Handle with tongs.
• Fill Dewar slowly using a transfer system.
• Decontaminate if leaks occur.

Emergencies

In case of exposure:

• Wash with warm water (≤42 °C).
• Do not rub frozen skin.
• Seek medical attention.

In case of spill:

• Evacuate the area.
• Report the incident.

👉 Form

Contact: comite.bioseguretat@uab.cat