Safety In the OR
ambulatory Licensed Competency 2026
Specimen Handling
Objectives
- Recognize proper handling of specimens and containers, including the use of gloves, appropriate labeling to prevent misidentification, and appropriate packaging for delivery to the destination lab (label appearance, additional information needing to be written on label, and the need for a requisition)
- Define the roles of the surgical team (circulator, scrub person, and surgeon) in ensuring accurate specimen naming and patient identification (including the two-person verification process between the scrub person and circulator)
- Discuss special circumstances with specimens that would require additional steps or specific processing considerations (muscle biopsies, prostate biopsies, etc.)
- Identify common errors in specimen handling, labeling, and verification, and apply strategies to prevent such errors through attention to detail, adherence to protocols, and teamwork
Laboratory Techniques
Specimen Handling Basics
- Save all specimens.
- If you are unsure if tissue is a specimen, ask the surgeon!
- Each specimen bag must contain only one specimen per bag (never mixing specimens from multiple patients in a single bag).
- Each specimen container must hold only one specimen.
- Every individual specimen container or tube must have a label adhered to it (not loose in the bag) or it will be rejected.
- Do not pre-label containers or tubes.
- Label only one container or tube at a time.
- Always complete the Post-op Checklist with the surgeon
- Among the items you will review together, ensure all specimens collected in the case are listed
Specimen Handling Basics
- The circulator and scrub person are responsible for performing positive patient identification and labeling the specimen correctly according to the name the surgeon provides
- This is ALWAYS a two-person verification between the circulator and the scrub person:
- The circulator creates the label
- The scrub person validates the specimen name and the patient information against the Flightboard before passing to the circulator
- The circulator immediately affixes the specimen label
Labels and Requisitions
- In eStar/Epic, do not sign an order until you are ready to deliver the specimen(s) listed in the order to the destination lab/dropoff point
- If a Lab Ready Labels (LRL) is written on, the lab will have to replace the label and this will delay the tests up to 5 hours
- LRLs are used for Clinical Pathology and Cytopathology.
FEL
Click here to view the Specimen Labeling/Requisition Comparison Table
VHBM
VSCF
Add Wilson County Surgery Center?
Special Situations
- Muscle biopsies are sent fresh to Anatomic Pathology
- Do not add saline to the specimen cup
- Do not put it in formalin
Special Situations
- Prostate biopsies are ordered using the "Prostate Biopsy" order under the Orders Preference List
- Review the Prostate Biopsy Specimens Tip Sheet for detailed steps
- Use pre-printed labelswhere available forstandard specimennaming conventions
- Indicate the name of thespecimen as well as thespecimen number for"Region of Interest"specimens
- Place biopsies in formalin
Try Again
Clarify the name of the specimen with the surgeon if there is a discrepancy. Then, correct the specimen label and all other documentation.
10
Interactive Question
11
Try Again
VHBM has a specimen lab on site. FEL delivers specimens to Anatomic Pathology on main campus. VSCF deposits specimens to a dropbox outside the facility.
12
Interactive Question
13
Try Again
A two-person verification is required (and moving over the sterile field is not ok).
14
Interactive Question
15
Try Again
All specimens except Clinical Pathology specimens (e.g., CBC, BMP, etc.) require a printed requisition.
16
Interactive Question
17
Try Again
For some Anatomic Pathology specimens, other orders may be used (e.g. Prostate Biopsy).
18
Interactive Question
19
Try Again
Numbering specimens only occurs in eStar/Epic but region of interest specimens need an additional name before it. On the physical specimen cup, a patient sticker is applied and only the specimen name is to be written.
20
Interactive Question
21
Try Again
Completing the Post-op Checklist is when the nurse reviews information (including specimens, wound class, and procedures performed) with the surgeon.
22
Interactive Question
23
Try Again
When testing occurs in different labs, there have to be separate specimens. It is appropriate to prompt the surgeon to send separate specimens if needed.
24
Interactive Question
25
Try Again
Clarify with the surgeon on the specimen name as needed, and do not hand off the specimen until patient sticker and specimen name are correct on the label.
26
Interactive Question
27
Try Again
Cultures and muscle biopsies are types of specimens that should never be introduced to formalin.
28
Interactive Question
29
References
[SOP] Handling of Surgical Specimens
[Policy] Submission of Surgical Specimens to Pathology
[Policy] Blood Product Administration
[VUMC2Go Tip Sheet] Collecting a Blood Bank Specimen - HR/PACU and Procedural Areas
[VUMC2Go Tip Sheet] Electronic Ordering of Labs
[Tip Sheet] Prostate Biopsy Specimens
Specimen Label & Requisition Comparison Table
Review: Will have to re-link due to PowerPolicy go-live after 10/27
30
Course Complete!
31
Specimens collected at VHBM are delivered to the on-site Specimen Lab and recorded in the log just inside
Not applicable for ambulatory sites.
Specimens collected at FEL are delivered to the adult inpatient Anatomic Pathology drop-off (3rd floor of the Vanderbilt University Hospital building) and recorded in the log at the window
Specimens collected at VSCF are placed in the drop box outsideThe leader must call the courier to retrieve the specimens
Ask the surgeon for the patient's clinical history
The specimen name for a Region of Interest is indicated where "xxx" is shown in the image.
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Transcript
Safety In the OR
ambulatory Licensed Competency 2026
Specimen Handling
Objectives
Laboratory Techniques
Specimen Handling Basics
Specimen Handling Basics
Labels and Requisitions
FEL
Click here to view the Specimen Labeling/Requisition Comparison Table
VHBM
VSCF
Add Wilson County Surgery Center?
Special Situations
Special Situations
Try Again
Clarify the name of the specimen with the surgeon if there is a discrepancy. Then, correct the specimen label and all other documentation.
10
Interactive Question
11
Try Again
VHBM has a specimen lab on site. FEL delivers specimens to Anatomic Pathology on main campus. VSCF deposits specimens to a dropbox outside the facility.
12
Interactive Question
13
Try Again
A two-person verification is required (and moving over the sterile field is not ok).
14
Interactive Question
15
Try Again
All specimens except Clinical Pathology specimens (e.g., CBC, BMP, etc.) require a printed requisition.
16
Interactive Question
17
Try Again
For some Anatomic Pathology specimens, other orders may be used (e.g. Prostate Biopsy).
18
Interactive Question
19
Try Again
Numbering specimens only occurs in eStar/Epic but region of interest specimens need an additional name before it. On the physical specimen cup, a patient sticker is applied and only the specimen name is to be written.
20
Interactive Question
21
Try Again
Completing the Post-op Checklist is when the nurse reviews information (including specimens, wound class, and procedures performed) with the surgeon.
22
Interactive Question
23
Try Again
When testing occurs in different labs, there have to be separate specimens. It is appropriate to prompt the surgeon to send separate specimens if needed.
24
Interactive Question
25
Try Again
Clarify with the surgeon on the specimen name as needed, and do not hand off the specimen until patient sticker and specimen name are correct on the label.
26
Interactive Question
27
Try Again
Cultures and muscle biopsies are types of specimens that should never be introduced to formalin.
28
Interactive Question
29
References
[SOP] Handling of Surgical Specimens
[Policy] Submission of Surgical Specimens to Pathology
[Policy] Blood Product Administration
[VUMC2Go Tip Sheet] Collecting a Blood Bank Specimen - HR/PACU and Procedural Areas
[VUMC2Go Tip Sheet] Electronic Ordering of Labs
[Tip Sheet] Prostate Biopsy Specimens
Specimen Label & Requisition Comparison Table
Review: Will have to re-link due to PowerPolicy go-live after 10/27
30
Course Complete!
31
Specimens collected at VHBM are delivered to the on-site Specimen Lab and recorded in the log just inside
Not applicable for ambulatory sites.
Specimens collected at FEL are delivered to the adult inpatient Anatomic Pathology drop-off (3rd floor of the Vanderbilt University Hospital building) and recorded in the log at the window
Specimens collected at VSCF are placed in the drop box outsideThe leader must call the courier to retrieve the specimens
Ask the surgeon for the patient's clinical history
The specimen name for a Region of Interest is indicated where "xxx" is shown in the image.