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Saxitoxina

Ema

Created on September 26, 2025

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Transcript

Toxicologia

Saxitoxina

Alexandre Costa, nº 68001 António Pires, nº 67774 Ema Belchior, nº 69124 Simão Pereira, nº 68246
G11 |

índice

9. Tratamento

1. Introdução

10. Diagnóstico

2. Análise estrutural

11. Prevenção

3. Mecanismo de ação

12. Prevenção

4. Efeitos terapeuticos/Potencial como droga

13. Impactos ambientais

5. Biosintese Molecular

14. Conclusão

6. Caso de estudo

15. Referências

7. Toxicidade

8. Sintomas

Introdução

  • A saxitoxina é uma toxina potente
  • Produzida por dinoflagelados marinhos e cianobactérias
  • Pertence às toxinas paralíticas do marisco (PSTs)
  • Bloqueia os canais de sódio dependentes da voltagem
  • Impedindo a entrada de iões e o consequente sinal

Análise estrutural

carbamato

guanidina

  • C10H17N7O4
  • Massa molar: 299,286 g/mol
  • Pertence à classe de alcalóides pirrolizidínicos e purínicos
  • Estrutura tricíclica
  • Elevada polaridade e hidrofilicidade

diól

Mecanismos de Ação

Interação do grupo guanidina da STX com um grupo carboxil.

Ligação ao canal não competitiva.

guanidina

Canal de sódio dependente de voltagem

A STX liga-se ao Sítio 1 da subunidade alpha do canal de sódio.

Inibe a formação e a propagação do potencial de ação.

Biossíntese Molecular

Dose Letal

1- 4

5,7

μg/kg

g/kg

Dose letal mínima em ratos
Dose letal mediana (LD50)
0,6 μg/kg
Dose letal mediana (LD50) por injeção
10 μg/kg
Dose letal mediana (LD50) em ratos

TOxicidade

15%

taxa de mortalidade
Anualmente ocoreem 2000 casos de PSP

Efeitos terapêuticos/ Potencial droga

Os canais de sódio são inibidos, sem causar morte celular.

Potencial uso como anastésico local.

Elevado nível de toxicidade e margem pouco segura

Uso tópico limitado

Mecanismos de difusão paracelular, alterações transitórias na permeabilidade intestinal e variação do estado de ionização com o pH

Rápida absorção

“Regra dos Cinco” de Lipinski

Descartada como terapêutico oral

Sintomas

Alguns dos sintomas incluem...

Vómito induzido

Intubação

Respiração mecanica

Lavagem gástrica

Fatores ambientais

Temperatura

Luz

Nutrientes

Simbiose

Afeta expressão dos genes sxtA e sxtG.Baixas temperaturas aumentam a expressão.

Intensidade luminosa determina o crescimento e metabolismo das algas.

Stress de nitrogénio inibe o crescimento de A. minutum. Mas escassez de fósforo aumenta a produção de STX. NO3- influencia a expressão dos genes sxt.

O zooplancton D. gessneri estimula os genes sxt de R. raciborskii. J. cystaugens induz stress oxidativo, nutritivo e sinais de quorum sensing.

DIagnostico e Prevenção

Controlo da piscicultura

Análise laboratorial

Evitar o consumo

A única maneira de averiguar corretamente a presença do contaminante é com a análise laboratorial.

Para assegurar que a qualidade do alimento é necessário confirmar as suas origens.

38 mg/kg

STX no mexilhão

Caso de estudo

0,8 mg/kg

Limite legal europeu

  • Em 2018 na Caparica
  • Dois indivíduos consumiram mexilhão pescado nas praias da Caparica
  • Continha STX produzida pela microalga Gymnodinium catenatum
  • Análises do plasma e urina por ensaios imunoenzimáticos (ELISA) e HPLC-FLD
  • Houve recuperação completa

200 g

mexilhão consumido

104 - 120 μg/kg

STX ingerida

100 μg/kg

Limiar de sintomas severos

Conclusão

Droga

Regulação das culturas e do consumo de marisco.

Prevenção

Saxitoxina

alvo

Sintomas

Produzida por dinoflagelados marinhos e cianobactérias

Uso seguro como anastésico tópico ocular.

Afeta o sistema nervoso e músculos, inibindo o potencial de ação por obstrução dos canais de sódio regulados por voltagem.

Desde formigueiro e dormência ligeira até paralisia muscular e insuficiência respiratória. Em casos descontrolados, potencialmente fatal.

veneno

A STX é perigosa e um problema para a segurança alimentar

Obrigada !

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- Author's Name

Structureyour content

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Timeline

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Demonstrate enthusiasm, sketch a smile and maintain eye contact with your audience can be your best allies when it comes to telling stories that excite and awaken the interest of the public: 'The eyes, chico. They never lie'. This will help you make a 'match' with your audience. Leave them open-mouthed!

Table + text

Demonstrate enthusiasm, give a smile, and maintain eye contact with your audience can be your best allies when it comes to telling stories that excite and arouse the audience's interest: 'The eyes, man. They never lie'. This will help you make a 'match' with your audience. Leave them open-mouthed!

Insert a video

Multimedia content is essential in a presentation to leave everyone amazed. Additionally, you will synthesize the content and keep your audience entertained.

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Although you should not abuse bulletpoints, icons and schemes can be great allies when telling stories.

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Audio

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This is a paragraph ready to contain creativity, experiences, and great stories.

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Conclusion

Disciplines like Visual Thinking facilitate visually rich note-taking thanks to the use of images, graphics, infographics, and simple drawings. Go for it!

Visual content is a cross-cutting language, universal, like music. We are able to understand images from millions of years ago, even from other cultures.

Did you know that Genially allows you to share your creation directly, without the need for downloads? Ready for your audience to view it on any device and give it exposure anywhere.

'Your content is liked, but it engages much more if it is interactive'-Genially

an awesome title here

With Genially's templates, you can include visual resources to leave your audience amazed. You can also highlight a specific phrase or data that remains engraved in the memory of your audience and even embed external content that surprises: videos, photos, audios... Whatever you want!

a great title here

With Genially's templates, you can include visual resources to leave your audience amazed. You can also highlight a specific phrase or data that will be etched in the memory of your audience and even embed external content that surprises: videos, photos, audios... Whatever you want!

an awesome title here

With Genially's templates, you can include visual resources to leave your audience amazed. Also, highlight a specific phrase or data that remains engraved in fire in the memory of your audience and even embed external content that surprises: videos, photos, audios... Whatever you want!

a great title here

With Genially's templates, you can include visual resources to leave your audience open-mouthed. Also, highlight some phrase or specific data that remains etched in your audience's memory and even embed surprising external content: videos, photos, audios... Whatever you want! Need more reasons to create dynamic content? Well: 90% of the information we assimilate comes to us through sight and, moreover, we retain 42% more information when the content moves.

  • Generate experiences with your content.
  • It has a WOW effect. Very WOW.
  • Make your audience remember the message.
  • Activate and amaze your audience.

a great title here

With Genially's templates you can include visual resources to leave your audience speechless. Also highlight some phrase or specific data that remains etched in the memory of your audience and even embed external content that surprises: videos, photos, audios... Whatever you want! Need more reasons to create dynamic content? Well: 90% of the information we assimilate comes to us through sight and, in addition, we retain 42% more information when the content moves.

  • Generate experiences with your content.
  • Has a WOW effect. Very WOW.
  • Make your audience remember the message.
  • Activate and amaze your audience.

a great title here

With Genially's templates, you can include visual resources to leave your audience amazed. Also, highlight a specific phrase or data that remains engraved in the memory of your audience, and even embed external content that surprises: videos, photos, audios... Whatever you want!

a great title here

With Genially's templates, you can include visual resources to leave your audience amazed. Also, highlight a specific phrase or data that remains etched in fire in the memory of your audience and even embed external content that surprises: videos, photos, audios... Whatever you want!

a great title here

With Genially's templates, you can include visual resources to leave your audience amazed. Also, highlight a specific phrase or data that remains etched in fire in the memory of your audience and even embed external content that surprises: videos, photos, audios... Whatever you want!

an awesome title here

With Genially's templates, you can include visual resources to leave your audience amazed. You can also highlight a specific phrase or data that remains engraved in the memory of your audience and even embed external content that surprises: videos, photos, audios... Whatever you want!

a great title here

With Genially's templates, you can include visual resources to leave your audience amazed. Also, highlight a specific phrase or data that remains engraved in fire in the memory of your audience and even embed external content that surprises: videos, photos, audios... Whatever you want!

a great title here

With Genially's templates, you can include visual resources to leave your audience amazed. Also, highlight a specific phrase or data that remains engraved in the memory of your audience and even embed external content that surprises: videos, photos, audios... Whatever you want!

a great title here

With Genially templates, you can include visual resources to leave your audience speechless. You can also highlight a specific phrase or specific data that will be engraved in the memory of your audience and even embed external content that surprises: videos, photos, audios... Whatever you want! Need more reasons to create dynamic content? Well: 90% of the information we assimilate comes to us through sight and, in addition, we retain 42% more information when the content moves.

an awesome title here

With Genially's templates, you can include visual resources to leave your audience amazed. You can also highlight a specific phrase or data that will be etched in the memory of your audience, and even embed external content that surprises: videos, photos, audios... Whatever you want!

a great title here

With Genially's templates, you can include visual resources to leave your audience amazed. You can also highlight a specific phrase or data that will remain engraved in the memory of your audience, and even embed external content that surprises: videos, photos, audios... Whatever you want!