Crime Scene Escape Room

Crime Scene Escape Room

Help Solve the Case

start

Introduction

Welcome to the Crime Scene Escape Room

Someone murdered Professor Apple in their home. Help crime scene investigators solve the crime using knowledge of two valuable techniques- Gel Electrophoresis and DNA fingerprinting.

Clues

Solve each clue: there is no other way to escape!

Clue 1

Clue 2

Clue 3

Clue 1

1/3

Watch the video: There are several items needed to make a gel. Which item is a salt water solution that will let electrical charges flow through the gel?

Buffer

Agarose

Clue 2

2/3

Next question:DNA is naturally _____________ charged. It will therefore travel toward the ______________ charge.

negative; negative

negative; positive

positive; negative

Clue 3

3/3

You're almost there.Recall the DNA sample in the video, what was the correct size of the smallest DNA fragment?

500

3500

1000

1500

Clues

Solve each clue: there is no other way to escape!

Clue 2

Clue 1

Clue 3

1/3

Clue 4

Watch the video:Use the restriction enzyme, EcoR1 to cut the sequence: GGAATTCCTTTCGGAATTCCATACG How many fragments will be produced?

four

DNA fingerprints can be produced by cutting DNA at specific locations to produce DNA fragments. Collectively these fragments represent a unique pattern. Watch the video to learn more about Restriction Enzymes.

three

two

2/3

Clue 5

Use the restriction enzyme, EcoR1 to cut the sequence below: GGAATTCCTTTCGGAATTCCATACG Count each letter to determine the size of the second fragment?

twelve

two

nine

3/3

Clue 6

Watch the video:Which suspect does the blood stain belong to?

Suspect 1

Watch the video concerning the technique DNA Fingerprinting. The first half of the video focuses on DNA Fingerprinting used in crime scene analysis

Suspect 2

Suspect 3

Clues

Solve each Clue: there is no other way to escape!

Clue 1

Clue 2

Clue 3

Clue 7

1/2

Take a good look at this image:Cut the DNA fragments for each suspect with EcoR1. Which suspect left genetic material at the crime scene? Suspect 1 GGGAATTCATACGAATTCTACGGAA Suspect 2 TCCCGGGATGAATTCCATACGA Suspect 3 ACGAGTTCCCCGAATTCAACTGAATTC

Suspect 1

Suspect 2

Suspect 3

Clue 8

2/2

Examine the Gel Results to determine who left DNA on the broken piece of flower planter. This was overlooked by the team and contains DNA that does not belong to the victim.

Alice

Chloe

Sara

Completed

Congratulations, you have successfully completed the escape room!

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