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Infografica Connettori Akihabara

FRANCESCO FERRERO

Created on November 29, 2024

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Transcript

INTRO

STEP 1

STEP 2

STEP 3

STEP 4

Ferrero, Talucci, Ruggia Pulito

STEP 4

  • observ the formation of a slightly trasparent reddish gelatinous skein (if you have used tomato it should be red)
  • with a glass rod try to take the skein to verify its costinstsency
  • in a petri dish try to solubilize the DNA in water

dolubilization of DNA in water

MATERIALS
  • Tomato
  • Extration solution (water and dish cleaner)
  • Citric acid (lemon juice)
  • Ethyl alcohol 95%
  • Becker, test tubes, dropper pipets
  • Funnel
  • Filter paper
  • Blender
  • Spinner

STEP 3

  • In a test tube put 2-3 cici of filtered a similar volume of ethyl alcohol make it go along the walls with the dropper to prevant their mixing

precipitation of DNA by insolubility in alcohol

gelatinous mass floating in gas during DNA aggregation

STEP 1

  • Cut two tomatoes into pieces
  • Blend the pieces of tomatoes (without skinless) with the extration solution
  • In a becker mix the smoothie with the salt solution and the lemon juice

membrans dissolution

protein denaturation (chromatine) protein agglutination

opaque coloured liquid containing various chemical components: cell remains (solid) and DNA (in solution)

STEP 2

  • filter the liquid obtined
  • if you want you can centrifuge and take the trasparent liquid in another test tube

separation of cellular remains from the liquid

clear-coloured DNA in solution