INTRO
STEP 1
STEP 2
STEP 3
STEP 4
Ferrero, Talucci, Ruggia Pulito
STEP 4
- observ the formation of a slightly trasparent reddish gelatinous skein (if you have used tomato it should be red)
- with a glass rod try to take the skein to verify its costinstsency
- in a petri dish try to solubilize the DNA in water
dolubilization of DNA in water
MATERIALS
- Tomato
- Extration solution (water and dish cleaner)
- Citric acid (lemon juice)
- Ethyl alcohol 95%
- Becker, test tubes, dropper pipets
- Funnel
- Filter paper
- Blender
- Spinner
STEP 3
- In a test tube put 2-3 cici of filtered a similar volume of ethyl alcohol make it go along the walls with the dropper to prevant their mixing
precipitation of DNA by insolubility in alcohol
gelatinous mass floating in gas during DNA aggregation
STEP 1
- Cut two tomatoes into pieces
- Blend the pieces of tomatoes (without skinless) with the extration solution
- In a becker mix the smoothie with the salt solution and the lemon juice
membrans dissolution
protein denaturation (chromatine) protein agglutination
opaque coloured liquid containing various chemical components: cell remains (solid) and DNA (in solution)
STEP 2
- filter the liquid obtined
- if you want you can centrifuge and take the trasparent liquid in another test tube
separation of cellular remains from the liquid
clear-coloured DNA in solution
Infografica Connettori Akihabara
FRANCESCO FERRERO
Created on November 29, 2024
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Transcript
INTRO
STEP 1
STEP 2
STEP 3
STEP 4
Ferrero, Talucci, Ruggia Pulito
STEP 4
dolubilization of DNA in water
MATERIALS
STEP 3
precipitation of DNA by insolubility in alcohol
gelatinous mass floating in gas during DNA aggregation
STEP 1
membrans dissolution
protein denaturation (chromatine) protein agglutination
opaque coloured liquid containing various chemical components: cell remains (solid) and DNA (in solution)
STEP 2
separation of cellular remains from the liquid
clear-coloured DNA in solution