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dna extraction

ALESSANDRO BERNOCCO

Created on November 29, 2024

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Transcript

DNA EXTRACTION

INSTRUMENTS NEEDED

01

03

05

DIFFERENT KINDS OF FRUITS

lemon juice(citric acid)

tubes

02

04

06

BLENDER

ethanol alcohol

sodium chloride

INSTRUMENTS NEEDED

09

11

07

funnel

dropper pipettes

becher

08

10

12

centrifuge

water

filter paper

steps

03

05

01

add alchohl by sliding it along the test tube's walls

with the funnel filter the fluid in a tube

blend the fruit with water in order to make a fluid

02

04

06

in the becher mix the fluid with lemon juice and salt

with the pipettes bring the gelatinous solution between two phases

split the solution in different tubes

observations

03

01

we added alchohl in order to precipitate the dna cause of the solubility of the substances

we added lemon juice and salt in order to denature and agglutinate proteins

04

02

the dna will be between the surnatant phase an the pellet

the fluid will be divided in supernatant solution (alcohol) and the pellet (fruit)