dna extraction
ALESSANDRO BERNOCCO
Created on November 29, 2024
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Transcript
DNA EXTRACTION
INSTRUMENTS NEEDED
DIFFERENT KINDS OF FRUITS
01
BLENDER
02
tubes
03
ethanol alcohol
04
lemon juice(citric acid)
05
06
sodium chloride
centrifuge
08
becher
09
water
10
funnel
11
filter paper
12
dropper pipettes
07
INSTRUMENTS NEEDED
steps
02
split the solution in different tubes
03
with the funnel filter the fluid in a tube
04
add alchohl by sliding it along the test tube's walls
05
with the pipettes bring the gelatinous solution between two phases
06
blend the fruit with water in order to make a fluid
01
in the becher mix the fluid with lemon juice and salt
observations
02
the dna will be between the surnatant phase an the pellet
03
we added alchohl in order to precipitate the dna cause of the solubility of the substances
04
we added lemon juice and salt in order to denature and agglutinate proteins
01
the fluid will be divided in supernatant solution (alcohol) and the pellet (fruit)