MAGIC BLUE BOTTLE EXPERIMENT

Bottle

Safety goggles

They protect the eyes from splashes of chemicals, flying particles, or radiation. They are crucial for preventing eye injuries and ensuring safe visibility during laboratory operations.

They provide a protective barrier for the hands against toxic, irritating, or contaminating substances. They are essential for preventing direct contact with chemicals and pathogens.

Gloves

Laboratory coat

It serves to protect the body from chemicals, biological agents, and other hazardous materials. Additionally, it keeps personal clothing clean and reduces the risk of contamination.

devices

PROTECTIVE

Personal

The objective of this experiment is to observe the oxygen behaviour and the redox reaction between glucose (C6H12O6) and methylene blue (C16H18CIN3S) in the presence of sodium hydroxide (NaOH), highlighting the phenomenon of the color change of the solution in an oxidation and reduction cycle.

EXPERIMENT

OBJECTIVE OF THIS

Notes

THEORY

EXAMPLE:

A redox reaction is a reaction in which electron transfer occurs between two c hemical species in the reaction.

REDOX

Reactions

Glucose is a simple sugar (a monosaccharide) that is one of the main sources of energy for the human body. Chemically it is a carbohydrate with the molecular formula C6H12O6.

GLUCOSE

Methylene blue is a chemical dye with formula C₁₆H₁₈ClN₃S, used as a redox indicator and biological dye. It changes color depending on the oxidation state: blue in an oxidizing environment and colorless in a reducing environment. An indicator is a substance that visually signals, often by a color change, a chemical property of a solution, such as pH or redox state.

METHYLENE

Sodium hydroxide, also known as caustic soda, is an inorganic chemical compound with the chemical formula NaOH. It presents as a white, solid and crystalline substance, highly hygroscopic (that is, it easily absorbs moisture from the air) and extremely corrosive.

Sodium hydroxide

• BEAKER
• SPRY BOTTLE WITH DISTILLED WATER (H2O)
• FLASK
• FLASK STOPPER

• METHYLENE BLUE (C16H18ClN3S)
• SODIUM HYDROXIDE (NaOH)
• GLUCOSE (C6H12O6)
• TECHNICAL BALANCE
• GLASS ROD

LABORATORY TOOLS:

PROCEDURE

Preparation of NaOH solution:Weigh 12 g NaOH and dissolve it in 50 ml distilled water in another beaker. Stir until completely dissolved.

STEP 2

Preparation of glucose solution:Weigh 1.2 g glucose and dissolve it in 100 ml distilled water in a beaker. Stir until completely dissolved.

STEP 1

PREPARATION OF SOLUTIONS

Observation of the reaction: - Initially, the solution will appear blue in color; - After 30-40 seconds, observe that the solution lightens to clear; - Shake the flask vigorously; the solution will take on a dark blue color; - Allow the flask to stand to observe the return of transparency.

STEP 2

Mixing of solutions: - Pour the 100 ml of the glucose solution into the flask; - Also add the 50 ml of the NaOH solution to the flask; - Add 2-3 drops of methylene blue; Close the flask with a stopper.

STEP 1

REACTION

DISCUSSION & RESULTS

Agitation of the flask provides oxygen to the solution environment, allowing methylene blue to regenerate. This process highlights the equilibrium dynamics of the redox reaction, where methylene blue can change from a colored to a colorless state and inversly, creating a visible and fascinating cycle.

DISCUSSION

The observed reaction is a clear example of oxidation-reduction: Oxidation: Glucose is oxidized to glucuronic acid (C6H10O7). Reduction: Methylene blue is reduced to a colorless form in the presence of sodium hydroxide (NaOH).

RESULTS

The “blue magic bottle” experiment not only illustrates the chemistry principles of oxidations and reductions, but also offers an interesting visual demonstration of chemical reactions. The system's ability to alternate between colors in response to physical (shaking) and chemical (presence of oxygen) stimuli is an excellent example of how chemical reactions can be influenced by environmental variables.

CONCLUSIONS

STEFANIZZI ALESSANDRO

SARNATARO MANILA

SANGES VINCENZO

ESPOSITO ALESSANDRO

DI GENUA EMMANUEL

CIPOLLETTA CRISTIAN

Team

THANKS for your ATTENTION !