MAGIC BLUE BOTTLE EXPERIMENT

Bottle

Personal

PROTECTIVE

devices

Gloves

Laboratory coat

They provide a protective barrier for the hands against toxic, irritating, or contaminating substances. They are essential for preventing direct contact with chemicals and pathogens.

It serves to protect the body from chemicals, biological agents, and other hazardous materials. Additionally, it keeps personal clothing clean and reduces the risk of contamination.

Safety goggles

They protect the eyes from splashes of chemicals, flying particles, or radiation. They are crucial for preventing eye injuries and ensuring safe visibility during laboratory operations.

OBJECTIVE OF THIS

EXPERIMENT

The objective of this experiment is to observe the oxygen behaviour and the redox reaction between glucose (C6H12O6) and methylene blue (C16H18CIN3S) in the presence of sodium hydroxide (NaOH), highlighting the phenomenon of the color change of the solution in an oxidation and reduction cycle.

THEORY

Notes

REDOX

Reactions

A redox reaction is a reaction in which electron transfer occurs between two c hemical species in the reaction.

EXAMPLE:

GLUCOSE

Glucose is a simple sugar (a monosaccharide) that is one of the main sources of energy for the human body. Chemically it is a carbohydrate with the molecular formula C6H12O6.

METHYLENE

Methylene blue is a chemical dye with formula C₁₆H₁₈ClN₃S, used as a redox indicator and biological dye. It changes color depending on the oxidation state: blue in an oxidizing environment and colorless in a reducing environment. An indicator is a substance that visually signals, often by a color change, a chemical property of a solution, such as pH or redox state.

Sodium hydroxide

Sodium hydroxide, also known as caustic soda, is an inorganic chemical compound with the chemical formula NaOH. It presents as a white, solid and crystalline substance, highly hygroscopic (that is, it easily absorbs moisture from the air) and extremely corrosive.

LABORATORY TOOLS:

• BEAKER
• SPRY BOTTLE WITH DISTILLED WATER (H2O)
• FLASK
• FLASK STOPPER

• METHYLENE BLUE (C16H18ClN3S)
• SODIUM HYDROXIDE (NaOH)
• GLUCOSE (C6H12O6)
• TECHNICAL BALANCE
• GLASS ROD

PROCEDURE

PREPARATION OF SOLUTIONS

STEP 1

STEP 2

Preparation of glucose solution:Weigh 1.2 g glucose and dissolve it in 100 ml distilled water in a beaker. Stir until completely dissolved.

Preparation of NaOH solution:Weigh 12 g NaOH and dissolve it in 50 ml distilled water in another beaker. Stir until completely dissolved.

REACTION

STEP 1

STEP 2

Observation of the reaction: - Initially, the solution will appear blue in color; - After 30-40 seconds, observe that the solution lightens to clear; - Shake the flask vigorously; the solution will take on a dark blue color; - Allow the flask to stand to observe the return of transparency.

Mixing of solutions: - Pour the 100 ml of the glucose solution into the flask; - Also add the 50 ml of the NaOH solution to the flask; - Add 2-3 drops of methylene blue; Close the flask with a stopper.

DISCUSSION & RESULTS

DISCUSSION

Agitation of the flask provides oxygen to the solution environment, allowing methylene blue to regenerate. This process highlights the equilibrium dynamics of the redox reaction, where methylene blue can change from a colored to a colorless state and inversly, creating a visible and fascinating cycle.

RESULTS

The observed reaction is a clear example of oxidation-reduction: Oxidation: Glucose is oxidized to glucuronic acid (C6H10O7). Reduction: Methylene blue is reduced to a colorless form in the presence of sodium hydroxide (NaOH).

CONCLUSIONS

The “blue magic bottle” experiment not only illustrates the chemistry principles of oxidations and reductions, but also offers an interesting visual demonstration of chemical reactions. The system's ability to alternate between colors in response to physical (shaking) and chemical (presence of oxygen) stimuli is an excellent example of how chemical reactions can be influenced by environmental variables.

Team

CIPOLLETTA CRISTIAN

DI GENUA EMMANUEL

ESPOSITO ALESSANDRO

SANGES VINCENZO

SARNATARO MANILA

STEFANIZZI ALESSANDRO

THANKS for your ATTENTION !