1.4.2 Vaccine Development

Vaccine Development

MI 1.4.2

RECOMBINANT DNA TECHNOLOGY

• cutting and recombining DNA fragments

• Can DNA from other species be combined? Why/why not?

BACTERIA AND RECOMBINATION

• In addition to a chromosome, bacteria have small, circular chunks of DNA called ______________
• These can be used as a vector to deliver genetic information

RECOMBINATION VIDEO

• Open PLTW 1.4.2 > Part II Recombinant DNA technology
• Watch Recombination Video
• DESCRIBE HOW THE TWO TOOLS OF MOLECULAR BIOLOGY—OFTEN DESCRIBED AS SCISSORS AND GLUE—ARE USED IN CONJUNCTION WITH PLASMIDS TO MAKE COPIES OF A GENE OF INTEREST.

• COMPLETE STEP 7 - SELF QUIZ

RESTRICTION ENZYME REVIEW

• enzymes that cut DNA at very specific sequences.

• See table in step 9 for common restriction enzymes & where they cut

• Sticky v. blunt ends

YOUR TURN!

• Engineer a plasmid that can produce a protein used to make a vaccine against the Hepatitis B virus.
• Make plasmid
• Underline/highlight all restriction sites in plasmid
• Refer to restriction rules in step 14 to decide where to cut open the plasmid & cut viral DNA
• Once you’ve decided on where to cut and insert, check with Ms. McAtee
• Insert viral DNA into plasmid and tape together to create recombinant DNA

• Answer conclusion questions