cellenONE HT

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Integration of cellenONE HT in a standard automated CLD platform

Read more about what cellenONE HT can do for Cell Line Development

Welcome

Head camera

Antistatic probe

Sample holders

PDC

Peristaltic pump

Service station

Syringe pump

Drop camera

Target holder

Axis system

Humidifier

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Environmental control

Watch cell isolation video

Watch fluorescence module

Plate Shuttle

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The plate shuttle is transferring the plates in and out of the cellenONE HT. The plate nest can host and secure the microtiter plates onto the shuttle during transport and dispensing.

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Incubator

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The standard automated CLD platform, comes with an automated incubator (Liconic STX44) where plates are stored on racks. The incubator is equipped with a barcode reader for generating the inventory and tracking the plates and an automated door to transfer the plates in and out. The size of the incubator is selected according to the throughput required. Temperature and CO2 level are controlled.​

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Robotic Arm

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In integrated workcells, e.g., standard or custom automated CLD platform, the collaborative robotic arm (Biosero PF400) is the limb of the system. It is equipped with finger-like grippers. The arm can precisely move and position plates for processing by the different devices.

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Application Programming Interface

cellenONE HT can be controlled in automated workcells through an API scheduler, which is the brain of the system. GreenButtonGo (Biosero) is implemented in the standard automated CLD platform. It is used to create, execute and monitor the workflow through a graphical interface ensures an intuitive user experience. Other schedulers may be used for integration in custom cellenONE HT automated platforms.

Cell Line Development

A scalable range of single cell solutions for Cell Line Development, from proof of concept to fully-automated large-scale bioproduction

Benefits of cellenONE HT

How cellenONE is revolutionizing CLD

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cellenONE Product Range

Cell Line Development

A scalable range of single cell solutions for Cell Line Development, from proof of concept to fully-automated large-scale bioproduction

Benefits of cellenONE HT

How cellenONE is revolutionizing CLD

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See Related Publications

cellenONE Product Range

• One SBS format holders.
• Compatible with 96, 384, 1536-well plates, proteoCHIP and microscope slides.
• Temperature controlled from 4 to 65°C.
• Fast temperature ramping up and down thanks to Qinstruments cold plates. (QInstruments)
• Software controlled according to sample preparation workflow requirement.

Target holder

• Imaging in PDC for cell sorting and isolation using brightfield and/or 4 channels fluorescence.
• Drop imaging and volume measurement.

Drop camera

• Two sample holder format.
• Compatible with Thermo Fisher Piko PCR Plate, 24-well plates / 96-well plates and 8-tube strips.
• Temperature control to improve cell viability and stability.

Sample holder

Precision

Autonomy

• Removing static charges to improve drop dispensing precision.
• Software controlled.

Antistatic Probe

• Supplying clean water to the PDC washing system.
• Collecting wastewater from the PDC washing system.

Peristaltic pump

Head Camera

• Vertical microscope mounted on the axis system.
• Pre-dispense scanning for target recognition and automated PDC positioning.
• Post-dispense imaging for quality control.

• Controlling humidity level inside cellenONE enclosure.
• Software controlled.

Humidifier

Environmental control probe

• Monitoring temperature and humidity inside the cellenONE deck.
• Controlling software communication to temperature and humidity devices to allow dew point operation.
• Enabling automated control of incubation steps in sample preparation workflows.

• Aspirating and/or flushing liquid through PDC.
• High precision sample uptake (from 1 to 200 µL).

Syringe pump

Its primary function is to monitor and regulate the environmental conditions inside the enclosure. By continuously measuring both temperature and humidity, this probe ensures optimal operating conditions for the system. Whether maintaining a specific temperature range for enzymatic reactions or controlling humidity levels to prevent sample desiccation, the probe contributes to the overall stability and accuracy of the cellenONE process.

Environmental control probe

• Allowing fast movement and precise PDC positioning.
• X-Y Linear Magnetic Drive.
• Precision <3 µm.

Axis system

Benefits

All versions of our cellenONE® platforms offer:

Accuracy

• Up to 100% single cell accuracy (no doublet, no empty well, no debris), leading to true single cell assays while reducing consumables and reagent consumption

Image-based technology

• User-defined sorting parameters (morphology and/or fluorescence markers) to select only cells of interest
• Increased assurance of monoclonality – fully documented with automated PDF report generation

Viability

• Ultra-gentle dispensing technology offers best-in-class clonal outgrowth on most cell lines

• Temperature control improves fragile cells’ viabilities (e.g. iPSC)

Versatility

• Any cell type from 0.5 μm to 80 μm
• Compatible with 96-, 384-, 1536-wp
• Multiple samples to multiple plates

Automation Cellenion provides both turnkey and custom solutions tailored to customers’ needs. Whether you want to integrate cellenONE® in your own automated system, or want to benefit from our scientific & automation expertise to set up together the ideal workflow for your cell line development, we have a solution for you!

• Glass capillary with piezo electric actuator.
• High precision and accuracy drop generation system.
• Drop volume from 150 to 600 pL.

PDC (Piezo Dispensing Capillary)

Precision

Autonomy