BCBI

PIAZZA Eleona

PRESENTAtion

Preparation of Blood Cancer Biobank Ireland for the Implementation of the ISO standard 20387:2018

start

Definition

What is a blood Biobank?

Distribution

tissue biobanks

population biobanks

blood

DNA

specialized repository

storage

collection

blood biobanks

digital biobanks

processing

PBMCs

research purpose

organoid biobanks

cell biobanks

RNA

What is a blood biobank?

INTRODUCTION

BCBI network

Dr. Eva Szegezdi, BCBI director

DUBLIN (3)

TULLAMORE

LIMERICK

WATERFORD

CASTLEBAR

GALWAY

CORK

9 leukemia treating hospitals

9 collection centers

2 storage centers

The BCBI collection

46%

Of the Samples biobanked are CLL

• 1 hospital added to the biobank network

• 817 patients recorded (mid july)

• 101 longitudinal samples (mid july)

Let's talk about the

OBJECTIVES

1a. Sample processing

UPMONT ARRIVAL IN THE BIOBANK

SOPs & documentation

CLASSIFICATION OF CONSENTS

BCBI code :

01-02-24

PT

AML

disease

center

First name Last name

DD-MM-YY

Clinical Data Retrieval

FIRST STEPS

one of the first task I was given to but time

93 patients :

• 65 AML samples with 5 longitudinal samples.
• 8 CLL samples
• 18 MM samples

GALWAY

Clinical Data Retrieval

INFORMATION NEEDED

- PID - Name - DOB - Gender - Sample collection date & time - Inital diagnosis - Cancer subtype - Diagnosis remarks - Disease history - Treatment response - Initial treatment - First treatment

one of the first task I was given to but time

Existing features

- Status (dead/alive)- WCC - Cytogenetics / karyotype / immunophenotyping - Blast count - Treatment date

New features

Clinical Data Retrieval

INFORMATION NEEDED

- PID

one of the first task I was given to but time

- Name - DOB - Gender - Sample collection date & time - Inital diagnosis

Existing features

- Cancer subtype - Diagnosis remarks - Disease history - Treatment response - Initial treatment - First treatment

- Status (dead/alive)

- WCC- Cytogenetics / karyotype / immunophenotyping - Blast count - Treatment date

New features

Clinical Data Retrieval

INFORMATION NEEDED

4. Clinical Data Retrieval

Organization & Management

IMPLEMENTATION OF NEW KEY FEATURES (1)

Before change request

After change request

Organization & Management

IMPLEMENTATION OF NEW KEY FEATURES (2)

Before change request

After change request

Processing of samples

Understand and learn the processing steps

1a. Sample processing

Understand and learn the processing steps

1a. Sample processing

Understand and learn the processing steps

1b. Sample processing

original workflow and r

1b. Sample processing

Improve its efficiency

Evaluation of sample quality

Internal audits

3. Quality Control

3 methods:

3. Quality Control

16 MNC samples

3. Quality Control

9 MNC samples

ISO 20387:2018

Biotechnology — Biobanking — General requirements for biobanking

ISO 20387:2018

Biotechnology — Biobanking — General requirements for biobanking

BCBI director

Dr. Eva Szegezdi

Haematologist at St. James Hospital / Trinity College

Consultant Haematologist at University Hospital Limerick

Haematologist at Midland Regional Hospital Tullamore

Haematologist at University Hospital Mayo

Haematologist at University Hospital Waterford

Haematologist at University College Cork

Haematologist at Mater Misericordiae Hospital

Haematologist at College Hospital Galway

Haematologist at Beaumont Hospital

Dr. Kanthi Perera

Dr. Maria Eduarda Couto

Dr. Michael O'Dwyer

Dr. Paul Browne

Dr. Ezzat ElHassidi

Dr. Mary Cahill

Dr. Peter O'Gorman

Dr. John Quinn

Dr. Ruth Cliford

Job Title

Job Title

Job Title

Job Title

Name Surname

Name Surname

Name Surname

Name Surname

12,5%

12,5%

50%

25%

Achievements

Over the 6-month internship

CONCLUSION

Some Improvements ?

ACKNOWLEGDMENTS

Shoumyadipta Kundu Micheál Ó Dálaigh Sharon Kelly Elzbieta KoronaAnsari Cliodhna Nolan

Eva Szegezdi Rossella Parrotta Leonie Durkan Javid Sabour Takanlu Wanshan Hu

Eduarda CoutoJanusz KrawczykDaina BaublytėMitchell KennedyPumla Bhekiwe Manyatsi

Thank you for your attention !

REFERENCES

• Moss PAH, Hoffbrand AV. 2011. Essential Haematology. 6th ed. Wiley-Blackwell.

• Park HS. What is new in acute myeloid leukemia classification? Blood Res. 2024 Apr 15;59(1):15. doi: 10.1007/s44313-024-00016-8. PMID: 38616211; PMCID: PMC11016528. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11016528/pdf/44313_2024_Article_16.pdf

• Ziath. 2024. Home Page. https://ziath.com

• ChemoMetec. 2024. NucleoCounter NC-200. https://chemometec.com/nucleocounters/nc-200/

• Irish Cancer Society. 2024. Chronic lymphocytic leukaemia (CLL). https://www.cancer.ie/cancer-information-and-support/cancer-types/leukaemia/chronic-lymphocytic-leukaemia-cll

• Ramirez J, Brown L, Thompson M, et al. 2022. Advances in chronic lymphocytic leukemia treatment. Front Oncol. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245471/

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