Bases de la PCR

Las bases de la PCR

01

02

Primers o cebadores

ADN polimerasa termoestable

Oligonucleotidos monocatenarios, cuyas regiones se quieren amplificar

Enzimas con actividad polimerasa a elevadas temperaturas

03

Desnaturalización

Separación de las dos cadenas, generando una molecula hibrida bicatenaria

04

Hibridación

Unión de dos moléculas por la complementariedad de bases originando molecula hibrida bicatenaria

05

Extensión

Tomando el ADN molde, se sintetiza la cadena complementaria y se parte del cebador

06

aL FINAL TENEMOS adn NUEVO

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