Janine's Medical Mystery

Janine's Medical Mystery

start

Introduction

Medical Mystery?

Meet Janine, a spirited 10th grader who lately, has been feeling off. What began as a simple sniffle quickly turned into something more serious. After visiting a doctor, she was handed a mysterious, bubbling solution and some surprising news: she has MRSA, a superbug. Janine needs your help to find a solution with her new solution!

Info

Click "info" to watch a short video about antibiotic resistance.

Watch the video in the introduction! Then type MRSA to continue!

Visit 1

1/3

Janine turns to WebMD and find that she has an antibitoic stock solution! We need to help her dilute this. What equation would we need to do a dilution?

M1V1=M2V2

PV=NRT

Visit 1

2/3

Great! We got it, we need to use the dilution equation. On the antibiotic stock solution we read that it has a concentration of 100 µg/mL. WebMD suggest we need a final concentration of 3 µg/mL and a final volume of 20 mL. How could we set-up this equation to find the missing volume?

(100µg/mL)(20mL)=(Xµg/mL)(3mL)

(100µg/mL)(XmL)=(3µg/mL)(20 mL)

(20µg/mL)(3mL)=(Xµg/mL)(100mL)

Visit 1

3/3

We're almost there. Solve for the missing volume!

(100µg/mL)(XmL)=(3µg/mL)(20 mL)

0.3 µg/mL

0.6 µg/mL

0.6 mL

10 mL

Janine decides to test the 0.6 mL of the 3 µg/mL antibiotic solution on some of her cells before she takes it! And all her cells, good and bad, instantly... DIE!!!

Doctor Visits

Examine each doctor's treatment plan and determine which solution is best to cure Janine.

Dr. Pepper

WebMD

Dr. Grey

Dr. House

1/2

Visit 2

Janine decide WebMD may not be the best solution. Her next visit is with Dr. Pepper. Dr.Pepper advises she use a different concentration! Calculate the new volume with the following information. M1 = 100 µg/mL V1 = x M2 = 0.5 µg/mL V2 = 20 mL

100 µg

0.1 mL

100 mL

2/2

Visit 2

If 0.6 mL of 3 µg/mL was too strong of a concentration that killed all her cells, predict what would happen with 0.1 mL of 0.5 µg/mL

The infected cells would not be killed AT ALL

The infected and good cells would ALL be killed

The infected cells would ALL be killed OVER TIME

Doctor Visits

Examine each doctor's treatment plan and determine which solution is best to cure Janine.

Web MD

Dr. Pepper

Dr. Grey

Dr. House

Visit 3

1/2

Knowing now that none of her infected cells would be killed, Janine has decided to visit the very well known Dr. Grey. She suggests you do some light reading on antibiotics

Click here when completed with the reading

Visit 3

2/2

Why is it important for scientists to find the optimal concentration of antibiotics?

To make sure the antibiotics are strong enough to kill all the bacteria.

To make sure the antibiotics are affordable for everyone.

To ensure that the antibiotics are effective without harming the patient.

Doctor Visits

Examine each doctor's treatment plan and determine which solution is best to cure Janine.

Web MD

Dr. Pepper

Dr. Grey

Dr. House

1/1

Visit 4

Janine now understand that she needs to find the optimal concentration and volume that won't kill all her cells, but in the correct amount of days will kill the bacteria. Her next doctor, Dr. House, suggest she calculate a few more concentrations and he can help her decide which is the best!

V1 = 0.02 mL

A. (100µg/mL)(XmL)=(0.1µg/mL)(20 mL)

V1 = 0.50 mL

B. (100µg/mL)(XmL)=(1.5µg/mL)(20 mL)

V1 = 0.30 mL

C. (100µg/mL)(XmL)=(2.0µg/mL)(20 mL)

V1 = 0.40 mL

D. (100µg/mL)(XmL)=(2.5µg/mL)(20 mL)

continue

Solución

Solution

CONGRATULATIONS

Dr. House checked your calculations and determined that the 2.0µg/mL was the best concentration. Janine has been taking her antibiotics for 3 days and feels much better

Start over?

Oh oh!

That answer is not correct...

But don't lose your balance, continue on your way and try again!

back

MRSA stands for methicillin-resistant Staphylococcus aureus. This bacterial infection is highly resistant to antibiotics (medicine). Watch the video to learn more.

1 - A

2- D

3 - B

4 - C