cellenONE-interactive

Welcome

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Syringe pump

Humidifier

Head camera

Peristaltic pump

Antistatic probe

Axis system

PDC

Fluorescence module

Environmental control

Drop camera

Inline degasser

Watch fluorescence module

Watch cell isolation video

Service station

Target holders

Sample holders

Watch source/target plate video

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Integrated electronic box to power the cellenONE

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About the technology

How it works

About cellenONE® X1 NEO

New Features

Applications

Single MicrobialCell Isolation

Cell Line Development

Single Cell Omics

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Single Cell Omics

Combining single cell isolation and nanoliter dispensing for sample preparation using a single device

scWG-Seq

See related publications

Spatial Proteomics

Proteomics

Zoom in on Proteomics Solutions

scRNA-Seq

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Single Cell Proteomics

Use cellenONE X1 Neo in combination with our proteoCHIP product range, for turn-key scProteomics solutions.

proteoCHIP 12*16

proteoCHIP EVO 96

proteoCHIP LF 48

See workflow

See workflow

See workflow

See related publications

Single Microbial Cell Isolation

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cellenONE is the optimal tool for sorting and isolation of microorganisms for both single cell omics analyses and culture-based applications

Applications

Key Features

See Related Publications

Benefits

A unique cell type range: any microbe, any size. Here is what we have tried so far, and we will be very happy to try other microorganisms that you would like to isolate!

High-throughput culturomics

Unique single cell accuracy

Image-based single cell isolation

Fungi

Prokaryotes

Use any target labware

Protozoa

Microphytes

Nanoliter liquid dispensing for reaction miniaturization

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Cell Line Development

A scalable range of single cell solutions for Cell Line Development, from proof of concept to fully-automated large-scale bioproduction

Benefits

Our range of products

How cellenONE is revolutionizing CLD

See Related Publications

PDC (Piezo Dispensing Capillary)

• Glass capillary with piezo electric actuator.
• High precision and accuracy drop generation system.
• Drop volume from 150 to 600 pL.

Benefits

Viability

• Ultra-gentle dispensing technology offers best-in-class clonal outgrowth on most cell lines

• Temperature control improves fragile cells’ viabilities (e.g. iPSC)

All versions of our cellenONE® platforms offer:

Accuracy

• Up to 100% single cell accuracy (no doublet, no empty well, no debris), leading to true single cell assays while reducing consumables and reagent consumption

Versatility

• Any cell type from 0.5 μm to 80 μm
• Compatible with 96-, 384-, 1536-wp
• Multiple samples to multiple plates

Image-based technology

• User-defined sorting parameters (morphology and/or fluorescence markers) to select only cells of interest
• Increased assurance of monoclonality – fully documented with automated PDF report generation

Automation Cellenion provides both turnkey and custom solutions tailored to customers’ needs. Whether you want to integrate cellenONE® in your own automated system, or want to benefit from our scientific & automation expertise to set up together the ideal workflow for your cell line development, we have a solution for you!

Humidifier

• Controlling humidity level inside cellenONE enclosure.
• Software controlled.

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Use any target labware

• Microorganisms can be isolated onto or into any consumable, MTP, petri dish, microscope slide, etc.
• Very high positional precision of cell isolation and reagent dispensing onto custom chips and microdevices

Service station

• Wash station for PDC cleaning.
• Waste station for sample discarding.
• Wash tray for PDC sterilisation.
• Holder for recovery tube.

Read app note

proteoCHIP LF 48

proteoCHIP LF 48 is an innovative solution for label-free single cell proteomics sample preparation. With its optimal design and accessories, it further extends the capabilities of cellenONE® single cell isolation technology. The combination of both technologies enables all the front-end of the mass spectrometry including incubation steps.

Benefits

• No vial exchanges, direct connection to the autosampler
• Data quality: identify and quantify >1000 proteins per single cell
• Clustering and differentiation of cell types

• Standardization: automated dispensing instead of manual pipetting enables more reproducibility
• Miniaturization: nanoliter reaction volumes increase sensitivity
• Throughput: prepare up to 144 single cells per run on cellenONE

Fluorescence Module

Cell imaging in PDC using fluorescence module (Blue, Green, Orange and Red)

• DAPI (ex.375nm; em. 432nm)
• FITC (ex.470nm; em.515nm)
• Cy3 (ex.565nm; em.580nm)
• Cy5 (ex. 625nm; em.670nm)

Microphytes

Autofluorescent or not, for instance: Alexandrium Minutum Thalasiossira Weissflogii Lauderia sp.

Target holder

• Two SBS format holders.
• Compatible with 96, 384, 1536 well plates, proteoCHIP and microscope slides.
• Temperature controlled from 4 to 65°C. ).
• Fast temperature ramping up and down thanks to Qinstruments cold plates.
• Software controlled according to sample preparation workflow requirement.

Syringe pump

• Aspirating and/or flushing liquid through PDC.
• High precision sample uptake (from 1 to 200 µL).

Sample holder

• Two sample holder format.
• Compatible with Piko PCR 24 and 96 plates and 8-tube strips.
• Temperature control to improve cell viability and stability.

High-throughput culturomics

Culture-based approaches are rising back in microbiology. Classical methods for isolate library generation are tedious and time-consuming, therefore increase in throughput is now needed. cellenONE can isolate any type of cells, and mixes of those types, including host and microbial cells in the same sample! With no need for fluorescent staining, cellenONE ensures optimal post-isolation cultivability, for abundant and diverse culture libraries.

Environmental control probe

Its primary function is to monitor and regulate the environmental conditions inside the enclosure. By continuously measuring both temperature and humidity, this probe ensures optimal operating conditions for the system. Whether maintaining a specific temperature range for enzymatic reactions or controlling humidity levels to prevent sample desiccation, the probe contributes to the overall stability and accuracy of the cellenONE process.

Image-based single cell isolation

User-defined sorting parameters (morphology and/or fluorescence) 4-channel fluorescence (DAPI, FITC Cy3, Cy5) Assurance of monoclonality: recorded images Immediate visual feedback on sample preparation (e.g. presence of host cells, debris, etc…) Link phenotypic and morphological information with omics data

Fungi

Filamentous fungi spores and conidia, and yeasts, for instance: Aspergillus Niger Aspergillus Oryzae Saccharomyces cerevisiae Botrytis cinerea

Unique single cell accuracy

Best-in-class single cell accuracy for all types of cells With our microLIFE software, cellenONE is now able to reach up to 100% single cells for all types of microbes (even the smallest ones), both in bright field and fluorescence: no equivalent on the market.

Learn more about microLIFE

Integrated eBox

• Powering cellenONE instrument
• Controlling cellenONE hardware

Prokaryotes

Bacteria and archaea, for instance: Bacillus Escherichia Lactobacillus Microccocus Pseudomonas Staphylococcus Haloferax

Axis system

• Allowing fast movement and precise PDC positioning.
• X-Y Linear Magnetic Drive
• Precision <3µm

Nanoliter liquid dispensing for reaction miniaturization

• Small droplet volume (150 to 600 pL)
• Compatible with a wide range of reagents (incl. culture media, molecular biology reagents…)
• Temperature and humidity control to prevent evaporation and enable on-deck incubations

Fresh/Waste water bottle tanks

• Supplying clean water to the the PDC washing system.
• Collecting waste water from the PDC washing system.

Environmental control probe

• Monitoring temperature and humidity inside the cellenONE deck.
• Controlling software communication to temperature and humidity devices to allow dew point operation.
• Enabling automated control of incubation steps in sample preparation workflows.

Drop camera

• Imaging in PDC for cell sorting and isolation using brightfield and/or 4 channels fluorescence.
• Drop imaging and volume measurement.

Peristaltic pump

• Supplying clean water to the PDC washing system.
• Collecting wastewater from the PDC washing system.

Protozoa

Dictyostelium Leishmania

Read app note

proteoCHIP 12*16

With its optimal design and accessories, the proteoCHIP 12*16 is an innovative solution that further extends the capabilities of cellenONE® technology by facilitating miniaturized sample preparation of individual cells for multiplex single cell proteomics workflows. It enables all the front-end of the mass spectrometry on cellenONE®, including incubation steps.

Benefits

• Throughput: isolate and prepare up to 576 single cells per run on cellenONE
• Recovery: direct injection via autosampler leads to maximum liquid recovery and very little peptide loss
• Data quality: identify and quantify >1500 proteins per single run, with outstanding signal-to-noise ratio (even with low or no carrier)

• Standardization: automated dispensing instead of manual pipetting enables more reproducibility
• Miniaturization: nanoliter reaction volumes save reagents costs and increase sensitivity
• Process safety: unique design and material prevents cross-contamination, while prefilling with oil overcomes evaporation

Inline degasser

• Integrated inline degassing system
• Preventing air bubble formation
• Ensuring drop stability

New Features

While cellenONE X1 is now recognized as the gold standard in flexible single cell platforms, with about 200 instruments installed globally, the new version, cellenONE X1 Neo, is even better, offering enhanced features and performance to meet your evolving needs.

• New heating / cooling system
• Better temperature control
• Faster ramping up and down
• Improved control of the incubation steps
• New optical system for increased fluorescence sensitivity
• Improved drop generation system for enhanced droplet stability
• Faster instrument start-up time
• New sample holder
• Allowing sample cooling
• New labware format (i.e. Thermo Scientific™ Piko PCR Plate 24-well , Piko PCR Plate 96-well, and 8-tube stripes)

• Application-dedicated software packages (e.g. proteomics, cloning, microbiology etc.)

• Improved user experience
• Automation of end-to-end sample preparation workflows

Read app note

proteoCHIP EVO 96

proteoCHIP EVO 96 is an innovative solution for label-free Single Cell Proteomics sample preparation. With its optimal design and accessories, it further extends the capabilities of cellenONE® single cell isolation technology. The combination of both technologies enables end-to-end miniaturized Single Cell Proteomics sample preparation for MS-based analysis. Using the proteoCHIP EVO 96 allows for single cell sample preparation directly on-deck of the cellenONE, including incubation steps. After sample preparation, the samples can be directly transferred into Evotips via centrifugation, allowing transfer to the Evosep One with no manual step.

Benefits

• Miniaturization: nanoliter reaction volumes for increased sensitivity
• Throughput: prepare up to 192 single cells per run on cellenONE (96 cells per chip, up to two chips per run)
• Clustering and differentiation of cell types

• End-to-end workflow in less than 3 hours
• Direct loading into Evotips via centrifugation
• Standardization: automated dispensing enables much superior reproducibility, compared to manual pipetting

Head Camera

• Vertical microscope mounted on the axis system.
• Pre-dispense scanning for target recognition and automated PDC positioning.
• Post-dispense imaging for quality control.

An open platform for microbial single cell omics

Antistatic Probe

• Removing static charges to improve drop dispensing precision.
• Software controlled.

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About cellenONE® X1 Neo

cellenONE® X1 Neo is a unique platform combining high accuracy single cell isolation and precision nanoliter reagent dispensing, allowing miniaturization of a range of single cell omics protocols and enabling automated cell line development workflows with best-in-class clonal outgrowth. This new version of the instrument brings new features which enhance performances and ease of use and will empower researchers working with single cells.

It enables

• Single cell sorting and isolation
• Miniaturized single-cell omics workflows
• Automated cell line development
• Processing of any sample (from just 1 cell up to 50,000 cells)