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Microscope
Lauren Cruz
Created on November 28, 2023
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Eyepiece
Objective Lens
Stage
Fine Knob
Coarse Knob
Stage Clip
Arm
Only write down the highlighted labels.
Notebook Template
Instructions for using a microscope.
An Easy to draw microscope
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thenounproject.com
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Basic Instructions for Using the Light Microscope Prepare the Workspace: Ensure you have a clean, well-lit, and stable surface for setting up the microscope. Keep the area organized and free from clutter. Turn On the Light Source: If your microscope has an adjustable light source, switch it on. Adjust the brightness to an optimal level for viewing the specimen. Place the Slide: Place the prepared slide on the stage of the microscope. Secure it in place using the mechanical stage clips or slide holder. Use the Lowest Objective Lens: Start with the lowest magnification objective lens (usually 4x or 10x) by rotating the nosepiece to position it over the slide. Adjust the Focus: Look through the eyepiece and slowly turn the coarse focus knob to bring the specimen into a rough focus. Then, use the fine focus knob for precise focusing. Continue adjusting until the image is clear. Change to Higher Magnification: If needed, switch to higher magnification lenses (40x or 100x) by rotating the nosepiece. Be cautious with higher magnifications as they have a smaller depth of field and require more precise adjustments. Center the Specimen: Adjust the mechanical stage controls to move the slide and center the area of interest within the field of view.
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- Start with the lowest magnification.
- Rotate the lenses to switch between them. They should "click" into place.
- If your image gets "lost" at a higher magnification, lower the magnifcation to find your image, and then try icreasing it again.
Objective Lenses (Magnification)
These lenses come in three different magnifications. 4x, 10x, and 40x. It can be rotated to switch between objective lenses. They should "click" into place.
coverslip
slide
- Place your specimen on a glass slide.
- Cover it with a coverslip.
- Be careful, but if anything breaks, make sure to let your instructor know immediately.
Preparing a specimen.
In order to view a specimen under the microscope you will need a slide and coverslip.