Student project presentation

Detection of Giardia Lamblia in Stool Samples

Graduation Project

Group members: Mariam Bettahar U20103312 Mais Mohammed Abdelrahman Abdallah Almasi U20100508 Joman Hisham Tayseer Mustafa U20100601

Supervised by:Prof. Raed Omar Abu Odeh

Index

6.

1.

Introduction

Materials & Methods

7.

2.

Results

Giardia lamblia

8.

Limitations

3.

Life Cycle

9.

Discussions

4.

Giardiasis

10.

5.

Conclusion

Our Aim

1. Introduction

Giardia lamblia, a microscopic parasite, is a leading cause of gastrointestinal infections worldwide. Understanding its biology and transmission is crucial for effective prevention and treatment strategies.

• Giardia lamblia, a microscopic parasite, is a leading cause of gastrointestinal infections worldwide.

• Understanding its biology and transmission is crucial for effective prevention and treatment strategies.

2. Giardia lamblia

3. Life Cycle

4. Giardiasis

• Severe Diarrhea
• Abdominal Cramps
• Nausea & Vomiting
• Bloating

• Increased gas
• Dehydration
• Burping
• Loose stools

• Microscopic Examination
• ELISA
• PCR
• q-PCR
• Duodenal Aspirate (Biobsy)

Symptoms

Symptoms

Diagnosis

Giardiasis

• Metronidazole
• Tinidazole
• Albendazole
• Benzimidazole
• Paromomycin
• Nitazoxanide

• Hygiene Practices
• Hand Washing
• Protecting Water Resources
• Proper Food Handling
• Proper Sewage Disposal

Prevention & Control

Treatment

Prevention & Control

• Watery Diarrhea
• Abdominal Cramps

Gas oatingPain

• Fatigue & Weakness
• Dehyration
• Weight Loss

5. Our Aim

QIAamp Powerfecal Pro DNA Kit (50)

Gel electrophoresis

Nanodrop

Omega Bio-Tek kit

BIO-RAD thermal cycler

UV light

"To employ molecular methods for sensitive detection of Giardia lamblia DNA in stool samples."

Research Workflow

DNA extraction from stool sample

Polyemerase Chain Reaction

UV light

Gel electrophoresis

Nanodrop

6. Materials and Methods

Materials

DNA sample manipulation for PCR analysis

DNA extraction from stool samples

PCR: sample+loading dye+ ladder marker

DNA concentration

PCR amplification

Electrophoretic bands

Method

Method

Method

Method

Method

Methods

Gel electrophoresis

BIO-RAD thermal cycler

Biological Safety Cabinet (BSC)

Observed under UV light

Nanodrop

Omega Bio-Tek kit

QIAamp Powerfecal Pro DNA Kit

7. Results

70 and 64 (+) for Giardia lamblia

Zero positive for Giardia lamblia

42 and 40 (+) for Giardia lamblia

12 (+) for Giardia lamblia

8. Limitations

Limitation

Consequence

01

Limited the extent of our investigation.

Shortage of available samples

02

DNA portion was not enough to do sequencing

DNA yield concerns

Prohibited us from expanding our data

03

Time (unexpected)

04

Successive samples had to be processed using the OMEGA BIO- Tek kit.

Reagent shortage of QIAamp powerfecal pro DNA kit

05

Unanticipated technical error with the bead beater

Vortexing at the expense of longer processing time

9. Discussions

8.3%

• Detected Giardia lamblia

• Achieved Amplification 240-250bp

• Identified Variations (5 out of 60) samples

91.7%

10. Conclusion

• Study highlights insights into molecular Giardia lamblia identification in stool samples, emphasizing genetic variability challenges.

• Acknowledges limitations and underscores the need for ongoing methodological refinement in giardiasis diagnostics and genetic exploration.

Thank You For Listening!

Any Questions ?

05:00

Cyst

• No flagella.
• Quadrinucleated.
• Oval or ellipsoidal in shape.
• Around 8 to 12 micrometers in diameter.
• Thick and resistant outer cyst wall.

Trophozoite

• 8 motile flagella.
• Binucleated.
• Pear shaped.
• 9 to 15 μm in length, , 5 to 15 μm in breadth, and 2 to 4 μm in thickness.
• adhesive disk on its ventral surface.