Student project presentation

Graduation Project

Supervised by:Prof. Raed Omar Abu Odeh

Group members: Mariam Bettahar U20103312 Mais Mohammed Abdelrahman Abdallah Almasi U20100508 Joman Hisham Tayseer Mustafa U20100601

Detection of Giardia Lamblia in Stool Samples

Conclusion

10.

Discussions

Results

9.

Materials & Methods

7.

Limitations

8.

Index

Giardiasis

5.

Our Aim

6.

Introduction

1.

2.

Giardia lamblia

3.

4.

Life Cycle

1. Introduction

• Giardia lamblia, a microscopic parasite, is a leading cause of gastrointestinal infections worldwide.

• Understanding its biology and transmission is crucial for effective prevention and treatment strategies.

Giardia lamblia, a microscopic parasite, is a leading cause of gastrointestinal infections worldwide. Understanding its biology and transmission is crucial for effective prevention and treatment strategies.

2. Giardia lamblia

3. Life Cycle

• Severe Diarrhea
• Abdominal Cramps
• Nausea & Vomiting
• Bloating

• Increased gas
• Dehydration
• Burping
• Loose stools

Prevention & Control

Symptoms

• Hygiene Practices
• Hand Washing
• Protecting Water Resources
• Proper Food Handling
• Proper Sewage Disposal

• Metronidazole
• Tinidazole
• Albendazole
• Benzimidazole
• Paromomycin
• Nitazoxanide

Diagnosis

Treatment

• Microscopic Examination
• ELISA
• PCR
• q-PCR
• Duodenal Aspirate (Biobsy)

Prevention & Control

Giardiasis

• Watery Diarrhea
• Abdominal Cramps

Gas oatingPain

• Fatigue & Weakness
• Dehyration
• Weight Loss

Symptoms

4. Giardiasis

"To employ molecular methods for sensitive detection of Giardia lamblia DNA in stool samples."

UV light

Gel electrophoresis

BIO-RAD thermal cycler

Nanodrop

Omega Bio-Tek kit

QIAamp Powerfecal Pro DNA Kit (50)

5. Our Aim

Nanodrop

UV light

Gel electrophoresis

Polyemerase Chain Reaction

DNA extraction from stool sample

Research Workflow

Nanodrop

Method

DNA concentration

Observed under UV light

Method

Electrophoretic bands

Gel electrophoresis

Method

PCR: sample+loading dye+ ladder marker

BIO-RAD thermal cycler

Method

PCR amplification

Biological Safety Cabinet (BSC)

Method

DNA sample manipulation for PCR analysis

Omega Bio-Tek kit

QIAamp Powerfecal Pro DNA Kit

Methods

DNA extraction from stool samples

Materials

6. Materials and Methods

12 (+) for Giardia lamblia

42 and 40 (+) for Giardia lamblia

Zero positive for Giardia lamblia

70 and 64 (+) for Giardia lamblia

7. Results

Vortexing at the expense of longer processing time

Successive samples had to be processed using the OMEGA BIO- Tek kit.

Prohibited us from expanding our data

DNA portion was not enough to do sequencing

Limited the extent of our investigation.

DNA yield concerns

05

Unanticipated technical error with the bead beater

Reagent shortage of QIAamp powerfecal pro DNA kit

Time (unexpected)

Shortage of available samples

04

03

02

Consequence

Limitation

01

8. Limitations

• Detected Giardia lamblia

• Achieved Amplification 240-250bp

• Identified Variations (5 out of 60) samples

91.7%

8.3%

9. Discussions

• Study highlights insights into molecular Giardia lamblia identification in stool samples, emphasizing genetic variability challenges.

• Acknowledges limitations and underscores the need for ongoing methodological refinement in giardiasis diagnostics and genetic exploration.

10. Conclusion

Any Questions ?

Thank You For Listening!

05:00

• No flagella.
• Quadrinucleated.
• Oval or ellipsoidal in shape.
• Around 8 to 12 micrometers in diameter.
• Thick and resistant outer cyst wall.

Cyst

• 8 motile flagella.
• Binucleated.
• Pear shaped.
• 9 to 15 μm in length, , 5 to 15 μm in breadth, and 2 to 4 μm in thickness.
• adhesive disk on its ventral surface.

Trophozoite