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Transcript
Bacterial Growth Media & Cultures
The Faces Behind Culture Media
Joseph Lister
Robert Koch
Richard Julius Petri
Joseph Schroeter
1860
The 1st liquid media is created
1878
The 1st pure culture of bacteria is taken
1881
The 1st isloated pure culture using a solid media was made
1887
The Petri Dish was created
- Tryptic Soy Broth
- Phenol Red Carbohydrate Broth
- MR-VP Broth
- Nutrient broth
- Hugh & Leifson's Oxidation Fermentation
- Stuart's & Amies Media
- Mannitol Motility Media
- Blood Agar
- Nutrient Agar
- McKonkey Agar
- Chocolate Agar
Semi-Solid
How are medias categorized?
Liquids
Solids
... or by the exact contents of the media
Synthetic Media
Basal Media
Complex Media
Enriched Media
Selective Media
Simple Media
McConkeyAgar
Mannitol Salts Agar
Differential or Indicator Media
Blood Agar
Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar
Summary of culturing bacteria
-- Founder of Antiseptic Medcine -- The 1st person to obtain a pure culture of a bacterium
Joseph Lister
-- Invented the glass Petri Dish -- Laboratory assistant to Koch
Richard Julius Petri
-- Developed liquid & solid forms of artifical media -- Discovered & described the Tuberculosis bacterium
Robert Koch
-- Discovered that chromogenic bacteria could grow on solid substrates
Joseph Schroeter
A list of common selective media and the bacteria they’re used to culture