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Bacterial Growth Media & Cultures

The Faces Behind Culture Media

Joseph Lister

Joseph Schroeter

Robert Koch

Richard Julius Petri

1881

1860

1887

1878

The 1st isloated pure culture using a solid media was made

The Petri Dish was created

The 1st pure culture of bacteria is taken

The 1st liquid media is created

How are medias categorized?

Liquids

Semi-Solid

Solids​

  • Tryptic Soy Broth
  • Phenol Red Carbohydrate Broth
  • MR-VP Broth
  • Nutrient broth
  • Hugh & Leifson's Oxidation Fermentation
  • Stuart's & Amies Media
  • Mannitol Motility Media
  • Blood Agar
  • Nutrient Agar
  • McKonkey Agar
  • Chocolate Agar

... or by the exact contents of the media

Enriched Media

Synthetic Media

Selective Media

Basal Media

Simple Media

Complex Media

Differential or Indicator Media

Mannitol Salts Agar

Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar

Blood Agar

McConkeyAgar

Summary of culturing bacteria

Joseph Lister

-- Founder of Antiseptic Medcine -- The 1st person to obtain a pure culture of a bacterium

Richard Julius Petri

-- Invented the glass Petri Dish -- Laboratory assistant to Koch

Robert Koch

-- Developed liquid & solid forms of artifical media -- Discovered & described the Tuberculosis bacterium

Joseph Schroeter

-- Discovered that chromogenic bacteria could grow on solid substrates

A list of common selective media and the bacteria they’re used to culture