BIOCHEMISTRY PRESENTATION
group 4
Dr.Julnar Usta
Dima ShehabDina Kabbara Jinane Samaha Zahraa Shokor Zahraa Atrisi
enzyme :
lysozyme
It's a technique used for determining the atomic and molecular structure of a crystal It has been associated with major breakthroughs
XRAY CRYSTALLOGRAPHY STEPS
xray
PROTEIN PURIFICATION
3rd structure obtained
1st
3rd
2nd
4th
data analysis
CRYSTALLIZATION
protein purification
Lorem ipsum dolor sit amet elit nibh.
crystallization
protein crystals
these are protein crystal locked with each crystal millions of protein molecules
X-Ray Diffraction
Determination Of Crystal Structures
Bragg's Equation
ny= 2d sin (theta)
Data Analysis
Processes
3.
1.
Electron densiy map is computed
The quality of the final product is checked with PROCHECK
4.
2.
The final structure is deposited into PDB (Protein Data Bank
Model building and refinement are conduced
The 3 Dimensional Structure Of A Protein
Obtaining protein crystals Recording the diffraction pattern Interpreting the data using a computer An atomic-resolution model of a protein
X Ray Sources and Detectors
X-Ray tube
Slide Types
Electrons responsibility in diffraction and intensity
Electrons scatter x-rays weaker than heavy metals
1.
Rotating anode tube
2.
Strong synchroton x-ray source
rotating anode
The First X-Ray Crystal Structure of a Full Length PAH Enzyme
Enim senectus tempor vivamus elementum gravida aptent quisque malesuada.
Biophysical Characterization
Of full length human PAH provides deeper understanding of its quaternary structure equilibrium (Between R and T form)
Pharmacological Chaperone
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer odio turpis, varius vitae tempor vel, venenatis vel lectus. Donec molestie, nisi in rhoncus faucibus, nibh dui elementum purus, at efficitur elit magna convallis mauris.
viral applications
understanding viral mechanisms
anti viral drug design and vaccine development
HIV /HIV protease
limitations
limited applicability
Radiation damage
Equipment and expertise
hydrogen atoms
Microcrystal Electron Diffraction
has enabled the determination of structures from very small crystals.
How to overcome the limitations?
SFX: serial femtosecond crystallogaphy
Room Temperature Serial Crystallography
to overcome radiation damages
Thank you!
Web links
References
https://www.immutoscientific.com/blog/x-ray-crystallography
https://www.rigb.org/explore-science/explore/person/william-lawrence-bragg-1890-1971
https://www.sciencedirect.com/science/article/abs/pii/S0165614704003050
https://medlineplus.gov/genetics/gene/pah/
https://www.nature.com/subjects/x-ray-crystallography/nmeth
https://pubmed.ncbi.nlm.nih.gov/28573569/
https://www.foxchase.org/news/2019-08-27-PAH-possible-treatment-strategies-pku
biochemistry presentation
Zahraa Shokor
Created on October 27, 2023
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Transcript
BIOCHEMISTRY PRESENTATION
group 4
Dr.Julnar Usta
Dima ShehabDina Kabbara Jinane Samaha Zahraa Shokor Zahraa Atrisi
enzyme :
lysozyme
It's a technique used for determining the atomic and molecular structure of a crystal It has been associated with major breakthroughs
XRAY CRYSTALLOGRAPHY STEPS
xray
PROTEIN PURIFICATION
3rd structure obtained
1st
3rd
2nd
4th
data analysis
CRYSTALLIZATION
protein purification
Lorem ipsum dolor sit amet elit nibh.
crystallization
protein crystals
these are protein crystal locked with each crystal millions of protein molecules
X-Ray Diffraction
Determination Of Crystal Structures
Bragg's Equation
ny= 2d sin (theta)
Data Analysis
Processes
3.
1.
Electron densiy map is computed
The quality of the final product is checked with PROCHECK
4.
2.
The final structure is deposited into PDB (Protein Data Bank
Model building and refinement are conduced
The 3 Dimensional Structure Of A Protein
Obtaining protein crystals Recording the diffraction pattern Interpreting the data using a computer An atomic-resolution model of a protein
X Ray Sources and Detectors
X-Ray tube
Slide Types
Electrons responsibility in diffraction and intensity
Electrons scatter x-rays weaker than heavy metals
1.
Rotating anode tube
2.
Strong synchroton x-ray source
rotating anode
The First X-Ray Crystal Structure of a Full Length PAH Enzyme
Enim senectus tempor vivamus elementum gravida aptent quisque malesuada.
Biophysical Characterization
Of full length human PAH provides deeper understanding of its quaternary structure equilibrium (Between R and T form)
Pharmacological Chaperone
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer odio turpis, varius vitae tempor vel, venenatis vel lectus. Donec molestie, nisi in rhoncus faucibus, nibh dui elementum purus, at efficitur elit magna convallis mauris.
viral applications
understanding viral mechanisms
anti viral drug design and vaccine development
HIV /HIV protease
limitations
limited applicability
Radiation damage
Equipment and expertise
hydrogen atoms
Microcrystal Electron Diffraction
has enabled the determination of structures from very small crystals.
How to overcome the limitations?
SFX: serial femtosecond crystallogaphy
Room Temperature Serial Crystallography
to overcome radiation damages
Thank you!
Web links
References
https://www.immutoscientific.com/blog/x-ray-crystallography
https://www.rigb.org/explore-science/explore/person/william-lawrence-bragg-1890-1971
https://www.sciencedirect.com/science/article/abs/pii/S0165614704003050
https://medlineplus.gov/genetics/gene/pah/
https://www.nature.com/subjects/x-ray-crystallography/nmeth
https://pubmed.ncbi.nlm.nih.gov/28573569/
https://www.foxchase.org/news/2019-08-27-PAH-possible-treatment-strategies-pku