The Scientist's Quest

BREAKOUT

's

Scientist

The

Quest

Start game

Story

© 2023 A game by The Sales Training Team

Legend says that a faraway kingdom forged an enzyme that together with the power of the Elemental Gems (Ex Taq, PrimeSTAR GXL, PrimeSTAR MAX, and the Catch All Gem) could amplify ANY DNA template. Gather the gems to assemble the ultimate mastermix and defeat the competitors hiding in the dungeon.

Start game

Thank goodness you’ve come! We need your help! You need to find the 4 Elemental Gems so that the enzyme has enough power and then defeat the competitors that are stealing our sales!

Help us, only a salesperson like you can do it!

Of course!

No thank you, I’ll pass

Ouch! I thought you were a cool salesperson. What will become of us!

Hehehe, I was kidding. Ahem, of course I’ll help you

I knew you would not fail me! Thanks a lot! Here is the holy pipettor. Now gather the Elemental Gems to complete it and defeat the competitors!

LEVEL 1 - Ex taq GEM

Ex taq GEM

Watch out, a wandering skeleton is in your way! Get the Ex Taq gem by passing a quiz about this enzyme!

Start level

Where do you think you’re going, mortal?! You won’t be worthy of the Ex Taq Gem until you get all my questions correct. Do you dare to try?

What exonuclease activity does Ex Taq have?

Both 5'-3 and 3'-5'

None

3'-5'

5'-3'

Takara Ex Taq polymerase offers high yield, excellent sensitivity, and fidelity that is ___ times higher than Taq polymerase.

10

20

4.5

Ex Taq DNA polymerase combines the proven performance of Titanium Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR.

False

True

Ex Taq was formulated to...

Amplify in the presence of inhibitors

Skip DNA extraction and purification

Tolerate a wide range of Mg2+ concentrations

Amplify GC-rich targets

Congratulations!! Here is your reward, you have earned it

Hahahaha, what do you say? ...

Oh, I've lost my mind, give me another chance!

LEVEL 2 - PrimeSTAR GXL GEM

PrimeSTAR GXL GEM

Choose the correct image to get out of the room alive and get the next gem

Start level

Which image best describes the formulation of PrimeSTAR GXL enzyme?

We can attribute PrimeSTAR GXL's processivity to ...

The "B" family structure

Proofreading ability

The "A" family structure

Elongation factor

The polymerases that are suitable for templates with GC content over 65% are..

Terra and PrimeSTAR GXL

PrimeSTAR MAX and PrimeSTAR GXL

LA Taq and PrimeSTAR GXL

Ex Taq and PrimeSTAR GXL

Which is not an application of PrimeSTAR GXL?

Library Prep

Regulatory gene regions

HLA Typing

Sequencing

RT-qPCR

Antibody engineering

Cloning

Polymorphic Regions

The novel PCR polymerase enzymes include

PrimeSTAR Series, LA Taq, Ex Taq

Titanium Taq, Advantage 2, Terra Taq

Ex Premier, Terra Taq, PrimeSTAR Series

Terra Taq, Ex Premier, Titanium Taq

You got the PrimeSTAR GXL Gem!

GAME OVER

Try again

Exit

LEVEL 3 - PrimeSTAR MAX GEM

PrimeSTAR MAX GEM

Drag the light to find the correct answer

Start level

All these are applications for PrimeSTAR MAX except...

Repetitive sequences

Fast PCR

RT-qPCR

Cloning

Antibody engineering

Sequencing

Which image best describes the formulation of PrimeSTAR MAX enzyme?

PrimeSTAR GXL will work with long, very complex, GC-rich targets and gives high fidelity. PrimeSTAR Max will also work on long targets with high fidelity, but is not as fast and cannot handle complex, GC-rich targets as well as GXL.

TRUE

FALSE

PrimeSTAR MAX has ____

Exonuclease activity of 3'-5' and creates T/A overhangs

Exonuclease activity of 3'-5'/5'-3' and creates T/A overhangs

Exonuclease activity of 3'-5/5'-3' and creates blunt ends

Exonuclease activity of 3'-5' and creates blunt ends

And now look for the PrimeSTAR MAX gem! Hurry up!

You got the PrimeSTAR MAX gem!

GAME OVER

Try again

Exit

LEVEL 4 - Catch all GEM

Catch all GEM

Search among the tiles for the mathematical operation to find the correct answer

Start level

20

8.5

30

24

17

19

27

Sum up the numbers: Advantage 2 can amplify plasmid targets up to __kb and gDNA targets up to _kb.

19

8.5

20

24

27

22

17

30

Our Terra product family enables you to perform PCR directly on tissue samples, crude extracts, or dirty samples. Terra polymerase is ideal for amplifying DNA targets up to 2 kb, even with GC content up to 70%.

TRUE

FALSE

The best enzyme for mitochondrial DNA and psuedogene identification is...

PrimeSTAR MAX

PrimeSTAR GXL

Titanium Taq

Ex Taq

Advantage 2

LA Taq

We can attribute Titanium Taq's ability to amplify complex DNA mixes by

N-terminal deletion to remove 5'-3' exonuclease activity

Optimized mutant Taq polymerase (amino acid substitution)

Hot-start anti-Taq antibody

The main differences between Advantage 2 and Titanium Taq is that Advantage 2 has

Better fidelity and added length capability

Better sensitivity and speed

Better fidelity and processivity

Better robustness and added length capability

You got the Catch All Gem!

GAME OVER

Try again

Exit

FINAL LEVEL - DRAGON

Defeat the dragon!

How dare you bother me, you fool!

THE END

You defeated the dragon, and the Takara kingdom is at peace again. And you’ve become became a legend!”

© 2023 A game by the Sales Training Team

What will become of us!

SURE YOU WANT TO GO OUT?

Stay

Exit