FBXW7
Kellie Krohn
Created on May 4, 2023
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Transcript
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Advanced Biochemistry Dr. Fishovitz Kellie Krohn
Protein Project: FBXW7
Why should we study cancer?
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Around 2,000,000 Americans are diagnosed with cancer each year. Around 600,000 Americans will pass away due to cancer in just one year.
Studying proteins may increase our understanding on what mechanisms drive cancer
Research
F-Box W7 Protein
belonging to the Skp1-Cdc53/Cullin-F-box-protein complex (SCF/β-TrCP)- F-Box proteins- regulate proteins involved in DNA replication, transcription, cell differentiation, and cell death, via ubiquitination-mediated degradation of target proteins (Kahn, 2021).
FBXW7 function
often inactivated or mutated in humans- correlates with:- cancer progression- therapy resistance - poor prognosis Indicated as a potential target for treatment for many cancers
FBXW7 role in humans
- Proliferation Assay- How a molecule may affect a cells proliferation, growth or viability
- Wound Scratch Assay- How a molecule affects migration of the cells
- Chemotaxis Assay- Whether cells migrate towards a particular agent
Cellular Assays
Research Techniques:
Endometrial Cancer Cell Lines
Scratch WoundAssay
Chemotaxis Assay
Proliferation assay
figure
Additional cell lines: HEC-1-B, HEC-1-A, KLE, SKOV3shRNA knockouts, western blots
Future Directions with studying FBXW7
The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China
FBXW7 role in:- Notch Signaling Pathway and Hepatic Fibrosis- Colorectal Cancer
Current Research on FBXW7
Eureka!
Thank you!!
Cuevas, I. C.; Sahoo, S. S.; Kumar, A.; Zhang, H.; Westcott, J.; Aguilar, M.; Cortez, J. D.; Sullivan, S. A.; Xing, C.; Hayes, D. N.; Brekken, R. A.; Bae-Jump, V. L.; Castrillon, D. H. Fbxw7 Is a Driver of Uterine Carcinosarcoma by Promoting Epithelial-Mesenchymal Transition. Proceedings of the National Academy of Sciences 2019, 116 (51), 25880-25890. https://doi.org/10.1073/pnas.1911310116.Liu, Y., Chen, H., Bao, H., Zhang, J., Wu, R., & Zhu, L. (2023). Comprehensive characterization of FBXW7 mutational and clinicopathological profiles in human colorectal cancers. Frontiers in oncology, 13, 1154432. https://doi.org/10.3389/fonc.2023.1154432Sun, Y., He, L., Guo, P., Li, F., Wang, B., Zhang, Y., An, K., & Peng, M. (2023). F-box and WD repeat domain containing 7 inhibits the activation of hepatic stellate cells by degrading delta-like ligand 1 to block Notch signaling pathway. Open medicine (Warsaw, Poland), 18(1), 20230634. https://doi.org/10.1515/med-2023-0634
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