Biochemistry ESCAPE ROOM

Biochemistry

Escape Room

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Introduction

"A group of 4 pre-med students walking through a dark street hear a call for help. They follow the noise which leads them into a suspicious house at the end of the block. The students wander through the house but end up locked in the home. After searching for an exit, one of the students finds a letter on the floor with a clue on how to escape. It reads, 'Correctly answer the series of questions if you want to be on your way to freedom.' The students put their giant brains together and start their journey..."

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Inventory

01

Suppose you are at dance practice learning a brand-new choreography and decide to eat some sunflower seeds for energy. This would be a good idea because:

Pyruvate dehydrogenase will be slowed down.

More sugar intake allows for glycogen formation.

Sunflower seeds are high in TPP.

02

If there is a lack of oxygen in muscle cells, resulting in oxygen depletion, the citric acid cycle would be affected. In which of these real-world examples would this occur?

Running on the treadmill which will cause glycolysis to undergo lactic acid fermentation instead of producing acetyl CoA

While drinking water which will cause the citric acid cycle to shut down in the moments you are not breathing.

While sleeping which results in the slowing down of all bodily functions.

03

A newborn baby presents to the Urgent Care with symptoms of eczema, recurrent illness, and tremors. Lab testing revealed that the patient has a disorder called Phenylketonuria which is characterized by a buildup of phenylalanine in the body. What could cause this condition to worsen?

The newborn baby has excess phenylalanine hydroxylase.

An excess of a-ketoglutarate dehydrogenase

An overexpression of isocitrate dehydrogenase.

04

Many different key enzymes participate in the citric cycle with one important one being citrate synthase. What would happen to the formation of citrate if the amino acid His320 on citrate synthase was changed to tryptophan?

Citrate will not form because the reaction mechanism will not proceed.

Citrate will form at a much faster rate due to the mutation.

Nothing will change since both amino contain similar properties.

It's not correct...

Find the key to continue

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Inventory

01

CHCHD10 mutations are known to be the root cause of fewer mitochondrial cristae junctions. If you were to have a patient with this mutation, which of the following side effects would you expect?

Overactive cellular functions from increased synthesis of ATP.

The patient would be unaffected.

Decreased cellular function due to a deficiency of ATP.

02

A patient comes in with a case of cyanide poisoning. The patient is treated with an antidote and fortunately lives. If no antidote existed how would the cyanide affect oxidative phosphorylation?

The electron transport chain will be inhibited not oxidative phosphorylation

It will cause inhibition of complex IV which will cause oxidative phosphorylation to also be inhibited resulting in cell death.

Cyanide poisoning will not affect oxidative phosphorylation, and everything will continue as normal.

03

Imagine you are an ecologist who is doing research on hibernating animals. You find that bears are rich in brown adipose tissue. What can you infer about the bear?

The bear’s main source of ATP will come from ATP synthesis

The bear will not undergo ATP synthesis

The bear contains a high amount of thermogenin

04

A female patient shows up to the Emergency room exhibiting cheilosis (scaling of the corners of the mouth), and extreme fatigue as one of the main symptoms. When asked about the patient’s health history, the patient mentions she is vegan, has developed gluten intolerance, does not take any multivitamins. Additionally, lab results revealed extremely low levels of vitamin B. Explain the correlation between vitamin B deficiency and the symptoms:

Vitamin B acts as coenzymes and long-term deficiency could also cause lactic acidosis in the patient.

B vitamins contain the necessary energy in form of ATP that your body needs

B vitamins are directly converted into glycolytic intermediates that then produce energy.

You found a secret message

♪♬ø

Remember these notes and play them on the piano in the correct order

01

...Keep playing

02

...One more

03

Try again...

01

When observing a patient who comes in after a sugar cookie eating contest, you discover that their blood glucose concentration levels are continuously increasing. Which of the following options could explain why this is occurring?

A mutation on the allosteric sites of phosphorylase A causing shifting from the T state

A mutation of phosphorylase A causing extreme sensitivity to glucose

A mutation on both phosphorylase A and B causing permanent R state configuration.

In which real-world example will phosphorylase kinase be activated due to the release of Ca2+ ions inactivating inhibitory 𝛿 subunit?

02

Sitting in class learning about activation of phosphorylase kinase

Lying in bed watching a movie

Lifting heavy weights at the gym

If a cell exhibited a mutation of synthase in which some fructose monomers were inserted into the glycogen base at the non-reducing end instead of glucose, what effect would this have on glycogen storage?

03

Glycogen storage would be less efficient.

There would be no effect on glycogen storage.

Glycogen storage would be inhibited.

The body goes through different steps to balance glucose levels after consuming a meal. The fed state is considered to be up to three hours after the consumption of a meal. The post-absorptive state is usually around 3-18 hours after consuming a meal. Which of the following statements are true regarding the post-absorptive state.

04

Glucagon stimulates liver cells to break down glycogen to be used as glucose source.

Epinephrine directly stimulates pancreas cells to synthesize glycogen.

High levels of insulin are secreted into the bloodstream to uptake the glucose.

You found a new item for your inventory

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Inventory

♪♬ø

Use the magnifying glass to find the code and then insert it into the typewriter.

224

Introduce el código secreto

Código(224)

A briefcase appeared...

congratulations

You've recovered all the ghost's memories! Now he can finally rest and you can escape from the house!