MUSICAL ROOM ESCAPE

ESCAPE BIOCHEM

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ESCAPE ROOM

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You need a little boost to your grade, so Mora decides to give you this project. Answer the questions to win the stars and reach an A in biochemistry!

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EXIT

Oxidative Phosphorylation

The Citric Acid Cycle

Glycogen Metabolism

EXIT

1/4

Suppose you have been working on a bacterial culture in the lab by altering its pyruvate dehydrogenase complex. You notice that one of bacterial culture is not growing correctly, this must have been because of a mutation you developed in the lab. Which of the following could have impaired the functioning of the pyruvate dehydrogenase complex, resulting in improper development of the bacteria?

The bacteria developed an iron deficiency due to the lack of citrate isomerization.

Hydroxyethyl-TPP forms, but Acetyl-CoA is not produced.

Enough glucose intake resulted in stimulation of Acetyl-CoA.

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2/4

As an essential intermediate in the citric acid cycle, oxaloacetate must be replenished frequently. Assume that the needs of the cell for oxaloacetate are greater than the amount available. What possible effect could this have on the body?

Heightened ATP levels, causing an increased rate of lipid synthesis.

Nucleotide synthesis would be disrupted, leading to the stoppage of DNA synthesis.

Increased rate of carbohydrate synthesis, leading to weight gain.

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3/4

You are currently working in a lab determining the effects of mutations on the active site of citrate synthase. While observing enzyme kinetic graphs, you notice one mutation causes a slight increase in Km when compared to the Wild Type. The enzyme is still functional. Which mutation most likely led to these results.

Asp 375 --> Glu 375

His 320 --> Gln 320

His 320 --> Lys 320

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4/4

Michael is an industrial worker who has been diagnosed with arsenite poisoning and is being treated with a medication containing a sulfhydryl reagent. Assuming Michael had not received his diagnosis, what would happen to Michael’s body from lack of intervention?

activity of PDC in the brain increases, worsening coordination and motor skills

elevation in lactic acid resulting in an increase in thiamine

His body is undergoing lactic acidosis

EXIT

Oxidative Phosphorylation

The Citric Acid Cycle

Glycogen Metabolism

EXIT

1/4

Imagine you put a donkey in a submarine, with low oxygen levels. What result would low oxygen levels have on this donkey’ cellular respiration’s ability to synthesize ATP?

The electron acceptor wouldn’t be present, resulting in cellular respiration coming to a stop.

The formation of a proton gradient would increase.

The remaining oxygen would be used in cytochrome c oxidase to bind iron and build excessive peroxide bridges between them.

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2/4

Monica is on a health & fitness journey and is gathering information for her biochemistry project on the effects of reactive oxygen species (ROS). She wants to know the effects of ROS in the body in conjunction with increased physical activity. Which of the following would happen?

Increased physical activity would decrease the concentration of ROS in the body, leading to a decrease in oxidative stress.

Increased physical activity would have no effect on ROS concentration.

Increased physical activity would increase the concentration of ROS in the body, leading to an increase in oxidative stress.

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3/4

The gamma subunit of ATP synthase is mutated, preventing the subunit’s rotation in a line of cultured cells. What effect does this have on the cell count.

Cell count increases

Cell count remains the same

The cell count decreases, however, some cells remain viable

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4/4

Calculate ΔG°’ for the following reaction under standard conditions: 2Cytochrome c (+3) + Ubiquinone (reduced) → 2Cytochrome (+2) + Ubiquinone (oxidized) +2H+ Cytochrome c (+3) +e- → Cytochrome c (+2) E’0 =0.22 V Ubiquinone (oxidized) +2 e- →Ubiquinone (reduced) E’0 = 0.10 V

-22.72 kJ mol-1

-102.26 kJ mol-1

-60.60 kj mol-1

EXIT

Oxidative Phosphorylation

The Citric Acid Cycle

Glycogen Metabolism

EXIT

1/4

Suppose that you are presented data that shows the affinity for the synthesis of glycogen. What are the effects of glycogen synthase staying in the R-state indefinitely?

Nucleotide synthesis would come to a halt.

The rate Kreb Cycle would increase.

Gluconeogenesis would produce excess of glyceraldehyde 6-phosphate.

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2/4

Mike is a professional soccer player that lives in sunny South Florida. One day after a three-hour practice in the hot sun, he passes out. After describing his symptoms and running tests, the on-site medic concludes that his body is not breaking down glycogen as it should. What could be happening here?

Glucagon levels are high, decreasing glycogenolysis.

Epinephrine levels are high, decreasing glycogenolysis.

Glucagon levels are low, decreasing glycogenolysis.

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3/4

Glycogen Storage Disease Type 1 (GSD-1) or von Gierke's Disease is caused by a mutation in glucose-6-phosphatase. Based on your understanding, which of the following is a likely symptom of this disease.

An increase in adipogenesis

Hypolipidemia

Lactic acidosis

EXIT

4/4

Anabella is riding her first roller coaster at an amusement park. While waiting in line, she becomes fearful and starts questioning her decision to get on the ride. How would activation of phosphorylase kinase be affected if Anabella continues to stand in line for the ride?

Increased activity due to excitatory emotions would stimulate protein kinase A

Inactivated enzyme would convert phosphorylase A to B

Muscle receptors are bound to hormone inhibiting phosphorylation

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CONGRATS!

You've answered all the questions and here's your well-deserved trophy! You've got biochemistry in your veins!

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