Biochemistry Escape Room - AH3

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Biochemistry

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Group AH-3 (Kamila Kemenyfy-Rojas, Vanessa Ponte, Christina Roca, Daniella Gonzalez)

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LEVEL 1

Chapter 17

LEVEL 2

Chapter 18

LEVEL 3

Chapter 21

QUESTION 1

Imagine a scientist plans on injecting cells with "detergent A". Following the injection, which of the following would occur in relation to cellular respiration? (Consider that the general cell membrane itself is not susceptible to detergent A; only the mitochondrial membrane is susceptible)

Cellular respiration would proceed as normal.

Both glycolysis and the citric acid cycle would take place.

Only glycolysis would proceed, and the process would come to a halt thereafter.

QUESTION 2

During the citric acid cycle, high-energy electrons are consumed to reduce NAD+ and FAD to NADH and FADH2. Imagine a person with a genetic disorder that impairs the function of NAD+ and FAD, leading to reduced ATP production. Which of the following statements best explains how this disorder affects the citric acid cycle?

The disorder will result in the accumulation of high-energy electrons, which will disrupt the citric acid cycle

The disorder will result in the accumulation of NAD+ and FAD, which will increase the rate of the citric acid cycle

The disorder will result in the reduced consumption of high-energy electrons, which will impair the function of the citric acid cycle

QUESTION 3

Multiple reactions can take place in the pyruvate dehydrogenase complex due to it being made up of multiple copies of each of the 3 enzymes. Suppose that a reducing agent is introduced to the system in surplus. How would the complex be affected?

There would be a surplus of unused lipoamide

The complex would not be able to continue its reactions to regenerate the oxidized lipoamide

The reactions would be unaffected since CoA is just a stoichiometric cofactor

QUESTION 4

The citric acid cycle is only done in aerobic conditions. How would living in a high-altitude place where oxygen is not abundant affect the cycle?

The citric acid cycle would proceed because the cycle would be able to complete itself just not at the same capacity as if oxygen levels were normal

There would be no cycle because oxygen is necessary for it to complete

The citric acid cycle would proceed because oxygen is not part of the net reaction of the cycle

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BACK

Chapter 17

LEVEL 1

Chapter 18

LEVEL 2

Chapter 21

LEVEL 3

The researcher creates three experimental groups where one group is exposed to oxygen in high concentrations, another group that has exposure to oxygen in low concentrations, and a last group that has typical exposure to oxygen in normal concentrations.

QUESTION 1

A researcher is studying the process of oxidative phosphorylation in mitochondria and wants to design an experiment to investigate the role of proton gradients in ATP synthesis. Which of the following experimental designs would be most appropriate?

The researcher manipulates the activity of cytosolic thermogenin. He creates 3 different groups, one group where the cells have upregulated activity of cytosolic thermogenin, a control group has a normal production of cytosolic thermogenin, and the third group which has higher expression of cytosolic thermogenin.

The researcher upregulates the production of mitochondrial thermogenin, which is a carrier protein that couples the flow of H to production of heat. He creates 3 different groups, one where the cells have a mitochondrial thermogenin knock out, a control group has a normal production of mitochondrial thermogenin and the third group will have higher expression than normal for this carrier.

QUESTION 2

The protein complexes generate ATP by phosphorylating ADP using energy from high-energy electrons

During a marathon, the muscles of the runners use oxidative phosphorylation to generate ATP for energy. Which of the following statements best describes the role of protein complexes in the electron transport chain during oxidative phosphorylation?

The protein complexes transfer high-energy electrons from NADH and FADH2 to oxygen, generating a proton gradient used to drive ATP synthesis

The protein complexes break down ATP to release energy that is used to transfer high-energy electrons to oxygen

QUESTION 3

Photosynthesis, passive diffusion

Q3 If the proton gradient wasn’t generated properly by the electron transport chain in the inner mitochondrial membrane, which of these processes would be disrupted?

Glycolysis, protein synthesis, photosynthesis

Glycolysis, protein synthesis

QUESTION 4

If one suffers from a mutation to complex IV, which one of these could be released as result?

O2

Carbon monoxide

Cytochrome c

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CONTINUE

BACK

LEVEL 1

Chapter 17

LEVEL 2

Chapter 18

Chapter 21

LEVEL 3

QUESTION 1

Consider a scenario where a person contracts a virus that is detrimental to the hydroxyl group of polar amino acids, resulting in an adverse conformational change of the structure of these amino acids. What repercussions would this have on the enzyme phosphoglucomutase?

Synthesis of phosphoglucomutase would occur at an accelerated rate.

Its active site would have an increased affinity for substrate.

Its active site would be damaged, thus preventing it from functioning.

QUESTION 2

During a long-distance race, the body relies on glycogen breakdown to provide energy for muscle contraction. A runner with low blood glucose levels might choose to consume a sports drink containing glucose and electrolytes to maintain their performance. How might the consumption of a sports drink affect the roles of glucagon and epinephrine in stimulating glycogen breakdown during the race?

The sports drink will decrease the release of glucagon and epinephrine, reducing glycogen breakdown and decreasing energy production

The sports drink will increase the release of glucagon and epinephrine, stimulating glycogen breakdown and increasing energy production

The sports drink will increase the release of glucagon, but decrease the release of epinephrine, leading to a shift in glycogen breakdown from muscle to liver

QUESTION 3

Think about the relationship between protein kinase A and protein phosphatase 1. How would the presence of protein kinase A affect glycogen degradation in the human body?

Glycogen degradation would decrease

Glycogen degradation would stay the same

Glycogen degradation would increase

QUESTION 4

A 55-year-old patient with diabetes is complaining of high blood sugar levels even though they routinely do insulin injections. When the bloodwork comes back, it is noted that the patient has high levels of protein kinase A. Why is this the case?

The patient is having an autoimmune response that is rejecting the insulin.

The patient is not giving themselves enough insulin

The patient is experiencing insulin resistance.

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