Escape Room #3 - GENETICS MYSTERY BREAKOUT

By: Christine Cunill

Mystery Breakout

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Intro

You have entered a dark and unknown world from which you have to get out before it's too late and you will be locked in forever. Can you escape with your knowledge of genetics?

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Mission 1

Locked

Locked

Locked

Answer the question to continue on

Given the following pedigree, what type of trait does it exhibit if the trait isn’t rare?

X-linked recessive trait

Autosomal dominant trait

X-linked dominant trait

Wronganswer

Y-linked trait

Answer the question to continue on

Amy is having a discussion with her friend Lola about X-linked dominant traits. Lola argues that if a female expresses the trait then all her children will also inherit the trait while Amy argues that not all their children will inherit the trait despite the mom expressing the trait. Who is right and why, if the trait in question isn’t rare?

A. Lola is correct because if the mom is homozygous then all her offspring will exhibit the trait.

B. Lola is correct because only females determine whether an X-linked trait is expressed.

C. Amy is correct because if the female is heterozygous then not all the kids will exhibit the trait.

D. Both A and C are correct

Answer the question to continue on

Given the following pedigree, what type of trait does it exhibit if the trait isn’t rare and the following information is given: Grandma’s genome is MM, Tony’s genome is mm and Lisa’s genome is MM?

Autosomal recessive trait

Autosomal dominant trait

Wronganswer

X-linked recessive trait

Y-linked trait

Answer the question to continue on

Which of the following statements is true about a pedigree given that the trait is Y-linked and is rare?

All male offspring of an affected male are affected

It appears only in males

Can be recessive or dominant

It doesn’t skip generations

Mission 2

Completed

Locked

Locked

Drag and discover

A scientist is trying to identify the murder suspect by using some DNA evidence found at the crime scene. The problem is that the scientist doesn’t have enough DNA to run tests, what can the scientist do to fix this problem?

Run it through a gel electrophoresis

Preform PCR on the given DNA

Preform Sanger’s Sequencing

Preform CRISPR-Cas9 on the sequence

Mission 3

Completed

Completed

Locked

Which of the following statements is false about the PCR process?

The purpose of annealing step is for the primers to anneal or join the separated DNA strands

There are 3 steps to PCR: denaturation, annealing, and DNA synthesis

The supplies for PCR are the DNA portion, a buffer, primers, DNA polymerase, and DNA nucleotides

The purpose of denaturation is to join the two DNA strands

Now that the DNA evidence has been through PCR, how will the scientist know if the DNA evidence was really copied?

Run it through a gel electrophoresis

Preform CRISPR-Cas9 on the sequence

25

Preform Sanger’s Sequencing

None of the following

Now that the scientist has more DNA from the crime scene, how is it possible for the scientist to find who the murderer is?

Because of DNA fingerprinting

Because of Sanger’s Sequencing

25

Because of non-homologous end joining

Because of CRISPR-Cas9

Remember the code

232

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Completed

Completed

Completed

Mission 4

Which of the following allows for differences in traits in offspring and generations?

B. Law of independent assortment

A. Law of segregation

D. Both A and B are correct

C. Product Law

In horses, a white coat is a trait coded by the genotype ww while a brown coat is a trait coded by the genotype WW or WW. If a female with the genotype ww crosses with a male with the genotype WW and their offspring have a white coat with brown spots what type of dominance is shown in this example?

Incomplete dominance

Complete dominance

Autosomal recessive trait

Codominance

A scientist is given the following mRNA sequence 5’CAUUGGACAGGU’3 that has been transcribed by DNA polymerase. What would be the coding or non-template DNA sequence of the given mRNA?

5’CATTGGACAGGT’3

5’GTAACCTGTCCA’3

3’CTAACCTGTCCA’5

3’GTAACCTGTCCA’5

Lily is studying an operon in her genetics lab, she notices that the operon is normally on but when a regulatory protein in this case a repressor is introduced the operon is no longer on. Given this information what kind of operon is Lily looking at?

Negative induced operon

Negative repressive operon

Positive repressive operon

Positive induced operon

4295

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