Cell transfection
By: Àngel Térmens Forcada
Start
Index
Introduction
Deliveries
Transfection types
Transfection methods
Reagents
Conclusion
01
Introduction to cell transfection
What is transfection?
Transfection is the process of introducing nucleic acids into eukaryotic cells by non viral methods.
How does transfection work?
Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment.
Transfection applications
...as one of the earliest study steps...
01
02
03
mammalian promoter and enhancer sequences studies
trans-acting proteins studies
mRNA processing
Transfection applications
...as one of the earliest study steps...
05
06
04
protein-protein interactions
translation studies
recombination events
02
Deliveries
DNA
Transcient and stable
RNA
Transcient
03
Transfection types
Transfection types
Stable transfection
Transient transfection
Cotransfection
Stable transfection
Transcient transfection
Cotransfection
04
Transfection methods
Transfection methods
Calcium phosphate transfection
RNAi & siRNA Transfection
Cationic lipid transfection
Transfection methods
CRISPR transfection
Electroporation
In vivo transfection
05
Reagents
Reagents for continuous cell lines
Neon electroporation
Lipofectamine 2000
Lipofectamine 3000
Lipofectamine LTX
Lipofectamine RNAiMAX
Lipofectamine CRISPRMAX
Invivofectamine 3.0
Reagents for primary and stem cells
Neon electroporation
Lipofectamine 3000
Lipofectamine Stem
Lipofectamine LTX
Lipofectamine RNAiMAX
Lipofectamine CRISPRMAX
Lipofectamine MessengerMAX
06
Conclusion
Conclusion
The choice between transient or stable transfection will usually depend on your goals, cell type and the facilities available to you. In short, transient transfection exerts a temporary influence on your cells, whereas stable transfection leads to permanent genetic changes that are usually passed on to future cell progeny.
Webgraphy
ThermoFisher scientist
Altogen Biosystems
Promega
Lonza
Thanks!
Transfection
Àngel Térmens Forcada
Created on June 12, 2022
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Transcript
Cell transfection
By: Àngel Térmens Forcada
Start
Index
Introduction
Deliveries
Transfection types
Transfection methods
Reagents
Conclusion
01
Introduction to cell transfection
What is transfection?
Transfection is the process of introducing nucleic acids into eukaryotic cells by non viral methods.
How does transfection work?
Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment.
Transfection applications
...as one of the earliest study steps...
01
02
03
mammalian promoter and enhancer sequences studies
trans-acting proteins studies
mRNA processing
Transfection applications
...as one of the earliest study steps...
05
06
04
protein-protein interactions
translation studies
recombination events
02
Deliveries
DNA
Transcient and stable
RNA
Transcient
03
Transfection types
Transfection types
Stable transfection
Transient transfection
Cotransfection
Stable transfection
Transcient transfection
Cotransfection
04
Transfection methods
Transfection methods
Calcium phosphate transfection
RNAi & siRNA Transfection
Cationic lipid transfection
Transfection methods
CRISPR transfection
Electroporation
In vivo transfection
05
Reagents
Reagents for continuous cell lines
Neon electroporation
Lipofectamine 2000
Lipofectamine 3000
Lipofectamine LTX
Lipofectamine RNAiMAX
Lipofectamine CRISPRMAX
Invivofectamine 3.0
Reagents for primary and stem cells
Neon electroporation
Lipofectamine 3000
Lipofectamine Stem
Lipofectamine LTX
Lipofectamine RNAiMAX
Lipofectamine CRISPRMAX
Lipofectamine MessengerMAX
06
Conclusion
Conclusion
The choice between transient or stable transfection will usually depend on your goals, cell type and the facilities available to you. In short, transient transfection exerts a temporary influence on your cells, whereas stable transfection leads to permanent genetic changes that are usually passed on to future cell progeny.
Webgraphy
ThermoFisher scientist
Altogen Biosystems
Promega
Lonza
Thanks!