Enzyme Activity Infographic

Enzyme Activity Experiment Analysis

Experiment Fundamentals

Methodology With Materials

The Variables

1. Firstly, gather and label 6 test tubes from A to F.
2. Utilize amylase solution and pour 1 cubic centimeter on each tube.
3. Then, collect other 6 test tubes and label them from 1 to 6, this is to differentiante the amylase from the starch solutions.
4. Consequently, pour 5 cubic centimeters of starch, in order to properly quantify the alterations of enzyme activity.
5. Seize 6 beakers and in to each pour different water temperatures at a proportion of 20ºC. You should have beakers containing temperatures of: 0ºC, 20ºC, 40ºC, 60ºC, 80ºC and 100ºC.
6. Then, group tube A with tube 1, then tube B with tube 2, and so on, proceeding to insert each group into the different beakers.
7. Let the solutions rest for 10 minutes, which assures both substances are on the same circumstances.
8. Meanwhile the tubes rest, grab a spot plate and insert iodine drops until the plate is fully filled. Use a second one just in case.
9. After the time ha passed, mix the solutions and begin to record the time
10. At two minute intervals,transfer a drop of each mixture into the iodine samples
11. Whenever the iodine remains brown, stop, because that's when all the starch from the mixture has been broken down.
12. Recollect results and present them in a table.

Independent:

• Starch Solution
• Temperature

Dependent:

• Amylase Activity

Experiment performed by Oxford University Press scientists from China, published in youtube channel "Oxford Mastering Biology" in 2020

Origins

Controlled:

• Amount. of substances used
• Time
• Beaker/Container used
• Iodine drops

Investigation and evaluation of temperatur'es impact over enzyme activity

Objectives

Research Question

How does amylase activity change with temperature?

Conclusion

Even though the experiment consisted of simple procedures measuring a basic concept of biology, in comparison of more complex areas regarding this field of study, it gives us the opportunity to reflect on the expansiveness that science holds. In just one particular topic, there's an extensive magnitude of areas that can be explored, and not only that, but what makes science unique is the quantity of approaches that exist for experimenting and proving a statement, an information, a hypothesis, a theory, and among other elements. In regards of this particular topic, we can arrive at the final conclusion that enzymes are a critical part for most reactions to occur and give result to important products, apart from the fact that there are distinct types of enzymes that react better at lower or higher temperatures than usual, for which this experiment can be catalogued as a general one, which helps to visualize the broad universe of biology.

Results Obtained

Result Analysis

The results gathered dictate a solid correlation between the temperature established and alterations of enzyme activity rate. It can be interpreted that enzyme reactions are either accelerated if the temperature increases, slowed down if the temperature decreases, or even completely stopped if the temperature surpasses the optimal limits. These conclusions can be affirmed by comparing the temperatures of 0ºC 40ºCºC and 100ºC. For instance, it's observable that 40ºC has an effective reaction rate because it's close to its optimum temperature, however, when exposed in extreme temperatures such as 0 ºC and 100ºC, we can see that enzyme activity has stopped, due that these completely disrupt the active site of enzymes, which prevents it from functioning correctly.

Elaborated by: Enrique Alejandro Amaya Villegas

Bibliography

Oxford Mastering Biology. (2020). "Practical 4.2 Investigation of the effect of temperature on enzyme activity". Oxford University Press. Retrieved from: https://youtu.be/ylhA84Uyb_A Cornell, B. (2016). "Enzyme Experiments". BioNinja: IB. Retrieved from: https://ib.bioninja.com.au/standard-level/topic-2-molecular-biology/25-enzymes/enzyme-experiments.html