UV-Vis Spectroscopy

UV-Vis Spectoscopy

Uses of Apparatus

Theory

• Medical Diagnostics
• Forensic Science
• Environmental Analysis
• Industrial
• Food Industry

Principle

Origin of Instrument

UV- Visible Spectroscopy follows the Beer-Lambert Law

Arnold Beckman, the founder of his eponymous company, introduced the DU UV-vis spectrophotometer in July 1941. It was the official version of the Model D prototype that he and Howard Cary had created.

Sample Handling Techniques

Basic Instrumentation

• Uv-visible spectra are usually recorded either in very dilute solutions or in the vapor phase.
• THe sample is dissolved in some suitable solvent which does not itself absorb radiation in the region under investigation.
• Commonly used solvents are cyclohexane, 1,4-dioxane, water and 95% ethanol.
• The chosen solvent should be inert to the sample.

There are 2 main types of spectrophotometer used to measure the absorption of U.V. or visible radiation: 1. Single beam UV-Vis. spectrophotometer 2.Double-beam UV-Vis. spectrophotometer

Instruments for measuring the absorption of U.V. or visible radiation are made up of the following components;

• Sources (UV and visible)
• Wavelength selector (monochromator)
• Sample containers
• Detector
• Signal processor and readout

Created By : Duwaun , Neecee, Lemuel & Luani

References : Tom, J. (2021, June 30). UV-Vis Spectroscopy: Principle, Strengths and Limitations and Applications | Technology Networks. Analysis & Separations from Technology Networks; Technology Networks. https://www.technologynetworks.com/analysis/articles/uv-vis-spectroscopy-principle-strengths-and-limitations-and-applications-349865 Spectrophotometry in 2021 | Tools for Applied Science | felixinstruments.com. (2019, May 15). Spectroscopy UV - Visible. https://felixinstruments.com/blog/spectrophotometry-in-2021/

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