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INFOGRAPHIC (HPLC)
ALAN ALEXIS TORRES MOTA
Created on September 9, 2021
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Transcript
High Liquid Chromatography Separationefficiency with positive ionization and detection using ion trap (hplc-ms / ms) can be used in the determination qualitative analysis of the main metabolites of some synthetic glucocorticoids: betamethasone, budesonide, dexamethasone, methylprednisolone, prednisolone and triamcinolone acetonide.
Qualitative determination of some metabolites of six glucocorticoids in urine through mass-coupled high-performance liquid chromatography.
The use of glucocorticoids isprohibited on the sports field during competitions if they are administered systemically, for which, WADA defined a level report of 30 ng / mL for parent compounds and their metabolites.
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It consists of the separation of components of a sample by means of the diverse physical and chemical interactions between the molecules and the particles of the column. The separated components are detected at the end of the column by a detector called a liquid chromatogram.
High Performance Liquid Chromatography (HPLC)