INFOGRAPHIC (HPLC)
ALAN ALEXIS TORRES MOTA
Created on September 9, 2021
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Transcript
High Performance Liquid Chromatography (HPLC)
It consists of the separation of components of a sample by means of the diverse physical and chemical interactions between the molecules and the particles of the column. The separated components are detected at the end of the column by a detector called a liquid chromatogram.
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The use of glucocorticoids isprohibited on the sports fieldduring competitions if they areadministered systemically,for which, WADA defined a levelreport of 30 ng / mL forparent compounds and theirmetabolites.
Qualitative determination of some metabolites of six glucocorticoids in urine through mass-coupled high-performance liquid chromatography.
High Liquid Chromatography Separationefficiency with positive ionization and detectionusing ion trap (hplc-ms / ms)can be used in the determinationqualitative analysis of the main metabolites of somesynthetic glucocorticoids:betamethasone, budesonide, dexamethasone,methylprednisolone, prednisolone and triamcinolone acetonide.