HERSHEY AND CHASE EXPERIMENT

Hershey and chase

EXPERIMENT

Ananya kushwahaB.Sc BT-ARCASC, Banglore

INTRODUCTION

According to earlier belives " Protein" is the genetic material not DNA.

AIM OF THE EXPERIMENT

To identify the fate of phage DNA after it exists the protein coat and enters bateria.

Hershey-Chase blender experiment

• Performed by Alferd Hershey and Martha Chase.
• Used E.coli as host and T-group Bacteriophage as pathogen

VIDEO

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T4 Phage was grown in two isotopic mediums containing :i) 35S - to label protein coat ii) 32P - to label DNA

Larger bacteria formed a solid pellet while viruses remained in supernatant.

The viral progeny was released as the bacteria were lysed.

Then the viruses and bacteria were separated via centrifugation.

Viruses were then allowed to infect the E.coli

OBSERVATION AND CONCLUSION

OBSERVATION :

• 32Phosphorus labelled Progeny of phages not 35sulfur

CONCLUSION :

• DNA is the genetic material.

References

1. https://www.washington.edu/

2. https://www.thermofisher.com/

3. https://hershey-chase.weebly.com/

4. https://fineartamerica.com/ etc.

THANKS