FOB Prac 2 activity 2

Preparing a wet mount of red onion skin peel

Use the dropper to place a small drop of water on the microscope slide.

Deionised water

Next

Preparing a wet mount of red onion skin peel

Good. Now time to add prepare the onion skin, click next

Next

Preparing a wet mount of red onion skin peel

Cut a small 1 x 1 cm slice of onion by clicking on the knife

Preparing a wet mount of red onion skin peel

Click the forceps peel a thin layer of cells from the onion slice as shown in the diagram.

Preparing a wet mount of red onion skin peel

Click the forceps to add the onion peel to the slide

Preparing a wet mount of red onion skin peel

Time to add a cover slip. Position a cover slip at an angle of 45 degrees at the edge of the water drop

Cover slips

Next

Preparing a wet mount of Hydrilla

Click on the cover slip to slowly lower onto the specimen

Preparing a wet mount of red onion skin peel

Great! Not even a trapped bubble. Time to view the specimen using the microscope. Click to place the slide on the microscope stage

Can't remember how to set up a microscope? - go to Prac 1

PREPARING A WET MOUNT OF ONION EPIDERMIS

Turgid cells Deionised water added to slide (Hypotonic solution) 40X objective

Draw a labelled scientific drawing of a few cells and send to your teacher

Next

Click to add saline solution to the slide.

Saline

Red Onion Epidermis (400X)

Next

Plasmolysed cells Saline solution added to slide (Hypertonic solution)

Draw a labelled scientific drawing of a few cells and send to your teacher

Next

RED ONION EPIDERMIS (400X)

Back to lab

Draw labelled scientific drawings of these cells and send to your teacher

Plasmolysed cells Saline solution added to slide (Hypertonic solution)

Turgid cells Deionised water added to slide (Hypotonic solution)