Hidden pigments in leaves
Leaf chromatography
Purpose
To identify plant pigments by separation and isolation of the pigments using thin layer paper chromatography.
Don´t mix different types of leaves. It is better to do the experiment with one type and then repeat with others different if you want to compare the results.
Materials needed
A mortar and pestle (mortero o similar)
Leaves
Coffee filters (papel de filtro)
A glass or container
Alcohol
Procedure-Steps
Cut the leaves in small pieces, put them in a mortar (or other container). Add a bit of alcohol and some sand (optional). Then grind them up.
Make sure you obtain enough green liquid, if not, add more alcohol and then filter it .
Now there are two options. You need filter paper (cofee filters can work)
Option 1: Fold in half the paper filter and place it vertical
Option 2: Fold the paper filter around a pen/pencil, the paper must touch the bottom of the container.
Leave it and wait until the pigments go up in the paper filter (30 min)
What pigments do you see in each leaf?
Pincha aquí para ver un guión de la práctica en español
Leaf chromatography
Marta
Created on October 2, 2020
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Transcript
Hidden pigments in leaves
Leaf chromatography
Purpose
To identify plant pigments by separation and isolation of the pigments using thin layer paper chromatography.
Don´t mix different types of leaves. It is better to do the experiment with one type and then repeat with others different if you want to compare the results.
Materials needed
A mortar and pestle (mortero o similar)
Leaves
Coffee filters (papel de filtro)
A glass or container
Alcohol
Procedure-Steps
Cut the leaves in small pieces, put them in a mortar (or other container). Add a bit of alcohol and some sand (optional). Then grind them up.
Make sure you obtain enough green liquid, if not, add more alcohol and then filter it .
Now there are two options. You need filter paper (cofee filters can work)
Option 1: Fold in half the paper filter and place it vertical
Option 2: Fold the paper filter around a pen/pencil, the paper must touch the bottom of the container.
Leave it and wait until the pigments go up in the paper filter (30 min)
What pigments do you see in each leaf?
Pincha aquí para ver un guión de la práctica en español