PhytoClear

• Goals:
• Purify zinc-polluted wastewater from industries

How ?

• Phytoremediation using Zinc hyperaccumulator plants
• Hydroponic system

REFS.

COLLAB.

ABSTRACT

Sup'Biotech Interactive Scientific Poster ©

• Total Budget: 626€

• Experiences: 3 months

BUDGET & TIMING

EXPECTEDOUTCOME

EXPERIMENTAL APPROACH

• New plant species, combinations and substrates
• Test at industrial scale
• Extend our solution to other sources of pollutants

• Assess whether selected plant species have zinc remediation activity on our system
• Identify the maximum zinc amount that a plant can accumulate
• Evaluate the influence of a substrate

CONTEXT

AIMS

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

• Matters:
• Global zinc mine production 2019: 13 million tons
• Reject in rivers and stream
• Deposit of polluted mud on banks and water acidity increasing.

PhytoClear

Environmental impact of Zinc industry

• Deposit of polluted mud on banks

• Water acidity increasing.

Zinc rejected in natural lac through industrial wastewater

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

• Global zinc mine production in 2019 estimated: 13 million tons.

CONTEXT

Incorporation and utilization of pollutants during plant growth

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

Phytoremediation

CONTEXT

• Bacteria capture organic matter and reject it in mineral

• Don't consume organic matter

• Plant = autotrophic organisms

Example of bacterial effect on nitrogen cylce

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

CONTEXT

Bacterial Mineralization

Bathed in "nutrient solution"

Plants are not seeded in the ground

Idealization of a basic hydroponic system

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

CONTEXT

Hydroponics

• Identify the maximum zinc amount that a plant can accumulate, depending on its species.

• Evaluate the influence of a substrate on the zinc uptake by selected plants.

• Identify the best remediation activity for zinc between different plant species.

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

• Assess whether selected plant species have zinc remediation activity on our system, by determining the abatement rate.

AIMS

• Model :

• Closed circuit of zinc-polluted water crossing over a hydroponic part (filtration)
• Two experiments:

• Determined the capacity of N. caerulescens and R. acetosa to diminish zinc concentration of a solution over time

• Added a substrate in the system to study its influence on bacteria development

System model diagram

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

EXPERIMENTAL APPROACH

• Model :

• Closed circuit of zinc-polluted water crossing over a hydroponic part (filtration)
• Two experiments:

• Determined the capacity of N. caerulescens and R. acetosa to diminish zinc concentration of a solution over time

• Added a substrate in the system to study its influence on bacteria development

System model diagram

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

EXPERIMENTAL APPROACH

For both experiments, several tracking will be executed weekly:

Abatement rate calculation

Colorimeter

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

• Sample twice a week
• Tools :
• Colorimetry method
• Abatement rate calculation

Zinc concentration and ionic solution parameters tracking

EXPERIMENTAL APPROACH

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

Measuring plant size using Image J

R. acetosa

N. caerulescens

PhytoClear

• Roots and aerial part evaluated weekly
• Non destructive analysis
• Tools :
• Software "Image J"
• Ended with a dry weight

Plant growth measurement

EXPERIMENTAL APPROACH

Example of serial dilution on Plate Count Agar

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

• Sampling the environment at the beginning and at the end of the experiment
• Tools :
• Plate Count Agar
• Macroscopic quantification

Bacteria quantification

EXPERIMENTAL APPROACH

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

Overview

• Budget estimation for the two first experiments.
• We included calorimeter depreciation expenditure (amortisation on 36 months).
• Total of 626€

PhytoClear

BUDGET

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

Details of expenditure for the three categories

BUDGET

• The second experiments will start at the end of october

• Once we prepared our system, the two experiments will be performed in the same way.

• These experiments include a week of preparation (assembly of structure, setting up of the circuit).

• For the following week, we need to have the plants ready to be used (we will need to have grown them).

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

TIMING

• Agricultural effluents
• Industrial sub-units (e.g. dairies).
• Other heavy metals.

3. Extend our solution to other sources of pollutants

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

• Get closer to the industrial scale.

2. Carry out experiments with larger volumes of zinc-polluted water

• Refine the R&D of our system.
• Increase the effectiveness.
• Perform additional testing.

1. Test others plant species and combinations as well as new substrates

EXPECTED OUTCOME

• (Lenntech.fr. 2020. Zinc, Propriétés Chimiques, Effets Sur La Santé Et L'environnement. Available at: <https://www.lenntech.fr/francais/data-perio/zn.htm#ixzz6KtyrjYbN>

• Rosenberg, E., 2015. “Heavy Metals in Water: Presence, Removal and Safety”. Johnson Matthey Technology Review, 59(4), pp.293-297. Burges, A., Epelde, L., Blanco, F., Becerril, J. and Garbisu, C., 2020.

• Ecosystem Services And Plant Physiological Status During Endophyte-Assisted Phytoremediation Of Metal Contaminated Soil. Clarholm, M., 1985. Interactions of bacteria, protozoa and plants leading to mineralization of soil nitrogen.

• Soil Biology and Biochemistry, 17(2), pp.181-187. France, H., 2020. Colorimètre De Poche DR300, Zinc, Avec Boîte | Hach France - Aperçu | Hach. Fr.hach.com. Available at: <https://fr.hach.com/colorimetre-de-poche-dr300-zinc-avec-boite/product?id=55097431489&callback=qs>

• Raviv, M. and Lieth, J., 2008. Soilless Culture. Elsevier. Senat.fr. 2020. Les Effets Des MéTaux Lourds Sur L'environnement Et La Santé. Available at: <https://www.senat.fr/rap/l00-261/l00-261_mono.html?fbclid=IwAR35eZYPBsW-yX_QQNg0ekeB8YsisEeyglEGT98lgAgqIkGWg3JRI1qoLoQ>

• SustainableDevelopment.un.org. 2020. Sustainable Development Goals .:. Sustainable Development Knowledge Platform. [online] Available at: <https://sustainabledevelopment.un.org/ menu=1300&fbclid=IwAR37SkvU9SxCC2iiIls6WWMa8joODi9dLdFPIz-RbzpfhXdIBHnf592RWMA

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

BIBLIOGRAPHY

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

PhytoClear is a project conducted by four Sup’biotech students in collaboration with the company Azuvia, that developed the filtrating greenhouse, a natural solution for natural pool water treatment based on phytoremediation, bacterial mineralization and hydroponics principles. Our objective is to demonstrate the suitability of Azuvia’s technology for zinc clearance from industrial effluents. To do so, we designed two water purification experiences with two plant species known to have zinc absorbing properties.

ABSTRACT

• School for engineers in Biotechnology,
• Offers an innovative training in 5 years after the baccalaureate in a large variaty sectors:
• Health and pharmaceuticals
• Food innovation
• Cosmetology
• Bioinformatics
• Environmenta

• Young company created in February 2019
• Develops eco-designed wastewater biological treatment solutions.
• Market: the private swimming-pool.
• The “Filtrating-Greenhouse”: purifies pool waters from organic pollutants by combining the following three main processes.

Our Partnerships:

Elie Altman, Eloïse Bicchieray, Maëlle Corosu, Capucine Mathieu

PhytoClear

COLLABORATIONS & ACKNOWLEDGEMENTS